NR1D1 Recombinant monoclonal antibody

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• NR1D1 Recombinant monoclonal antibody (82699-7-RR)

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  WB analysis using 82699-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82699-7-RR (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82699-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82699-7-RR against Human NR1D1 were performed. The affinity constant is below 1 pM.

NR1D1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 82699-7-RR

Clone No.251520G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ear 1, EAR1, EAR-1, HREV, Rev erbA alpha

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, A549 cells, NIH/3T3 cells, Mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82699-7-RR targets NR1D1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5964

  Product name: Recombinant human NR1D1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC056148

  Sequence: MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQR

• Full Name: nuclear receptor subfamily 1, group D, member 1
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC056148
• Gene Symbol: NR1D1
• Gene ID (NCBI): 9572
• RRID: AB_3743414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20393
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.