NR2F1 Polyclonal antibody

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  WB analysis using 24573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24573-1-AP (NR2F1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24573-1-AP

  WB result of NR2F1 antibody (24573-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR2F1 transfected HEK-293 cells.

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  IHC staining of human stomach cancer using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24573-1-AP (NR2F1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24573-1-AP (NR2F1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24573-1-AP (NR2F1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24573-1-AP (NR2F1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

NR2F1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24573-1-AP

Publications(7)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

COUP TF I, COUP TF1, COUP TFI, COUP transcription factor 1, COUP transcription factor I, EAR 3, EAR3, ERBAL3, NR2F1, NR2F2, SVP44, TCFCOUP1, TFCOUP1, V erbA related protein 3

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells, NIH/3T3 cells
• Positive IHC detected in: human stomach cancer tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

24573-1-AP targets NR2F1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR2F1 fusion protein Ag0118
• Full Name: nuclear receptor subfamily 2, group F, member 1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC004154
• Gene Symbol: NR2F1
• Gene ID (NCBI): 7025
• RRID: AB_2879616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10589
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR2F1 (Nuclear receptor subfamily 2, group F, member 1), also known as COUP-TFI, is an orphan nuclear receptor belonging to the superfamily of steroid/thyroid hormone receptors and acting as a strong transcriptional regulator. NR2F1 has a DNA binding domain (DBD) consisting of two conserved Zinc-finger motifs and a ligand-binding domain (LBD). NR2F1 also plays an epigenetic role in mediating global histone modifications by interacting with or recruiting chromatin-remodeling enzymes. NR2F1 can be a biomarker of tumor dormancy that promotes quiescence of various types of cancer cells, including breast, prostate, and squamous cell carcinoma of the head and neck (PMID: 35740627).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IHC	J Exp Clin Cancer Res; Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma.; Authors - Xiao Yang
human	WB,IF	Cell Death Dis; Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.; Authors - Meng Zhang
human	WB,IHC	Cancers (Basel); NR2F1 Regulates TGF-β1-Mediated Epithelial-Mesenchymal Transition Affecting Platinum Sensitivity and Immune Response in Ovarian Cancer; Authors - Qiuju Liang ; KD Validated
human	WB	Clin Transl Med; Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma; Authors - Hao Zhang
human	WB	Transl Oncol; Hypoxia induced cell dormancy of salivary adenoid cystic carcinoma through miR-922/DEC2 axis; Authors - Li Dai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina

NR2F1 Antibody Immunohistochemistry validation (1:100 dilution) in zebrafish retina (Cat no:24573-1-AP)