NR2F1 Polyclonal antibody

NR2F1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24573-1-AP

Synonyms

COUP TF I, COUP TF1, COUP TFI, COUP transcription factor 1, COUP transcription factor I, EAR 3, EAR3, ERBAL3, NR2F1, NR2F2, SVP44, TCFCOUP1, TFCOUP1, V erbA related protein 3



Tested Applications

Positive WB detected inhuman placenta tissue, NIH/3T3 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24573-1-AP is NR2F1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR2F1 fusion protein Ag0118
Full Name nuclear receptor subfamily 2, group F, member 1
Calculated molecular weight 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC004154
Gene symbol NR2F1
Gene ID (NCBI) 7025
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NR2F1 antibody 24573-1-APDownload protocol
IHC protocol for NR2F1 antibody 24573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
humanIHC

J Exp Clin Cancer Res

Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma.

Authors - Xiao Yang
humanWB,IF

Cell Death Dis

Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.

Authors - Meng Zhang
humanWB,IHC

Cancers (Basel)

NR2F1 Regulates TGF-β1-Mediated Epithelial-Mesenchymal Transition Affecting Platinum Sensitivity and Immune Response in Ovarian Cancer

Authors - Qiuju Liang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryan (Verified Customer) (06-15-2018)

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: zebrafish retina
NR2F1 Antibody Immunohistochemistry validation (1:100 dilution) in zebrafish retina (Cat no:24573-1-AP)