|Positive WB detected in||fetal human brain tissue, COLO 320 cells|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10975-2-AP targets Nurr1/NR4A2 in WB, IHC, IF, ChIP,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Nurr1/NR4A2 fusion protein Ag1418|
|Full Name||nuclear receptor subfamily 4, group A, member 2|
|Calculated molecular weight||66 kDa|
|Observed molecular weight||66 kDa|
|GenBank accession number||BC009288|
|Gene ID (NCBI)||4929|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].
Eur Rev Med Pharmacol Sci
Correlation between Nurr1 expression and drug resistance in the brain of rats with epilepsy.
Mol Biol Rep
Pramipexole attenuates 6-OHDA-induced Parkinson's disease by mediating the Nurr1/NF-κB pathway.
Exp Ther Med
Wnt1 silencing enhances neurotoxicity induced by paraquat and maneb in SH-SY5Y cells.
Exp Ther Med
Attenuated macrophage activation mediated by microRNA-183 knockdown through targeting NR4A2.
Int J Biochem Cell Biol
Caveolin-1 facilitates cell migration by upregulating nuclear receptor 4A2/retinoid X receptor α-mediated β-galactoside α2,6-sialyltransferase I expression in human hepatocarcinoma cells.
miR-206 modulates lipopolysaccharide-mediated inflammatory cytokine production in human astrocytes.