Nurr1/NR4A2 Polyclonal antibody

Nurr1/NR4A2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10975-2-AP

Synonyms

HZF 3, NOT, NR4A2, NURR1, Nurr1/NR4A2, Orphan nuclear receptor NURR1, RNR1, TINUR



Tested Applications

Positive WB detected infetal human brain tissue, COLO 320 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10975-2-AP targets Nurr1/NR4A2 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nurr1/NR4A2 fusion protein Ag1418
Full Name nuclear receptor subfamily 4, group A, member 2
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC009288
Gene symbol NR4A2
Gene ID (NCBI) 4929
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].

Protocols

Product Specific Protocols
WB protocol for Nurr1/NR4A2 antibody 10975-2-APDownload protocol
IHC protocol for Nurr1/NR4A2 antibody 10975-2-APDownload protocol
IF protocol for Nurr1/NR4A2 antibody 10975-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

Eur Rev Med Pharmacol Sci

Correlation between Nurr1 expression and drug resistance in the brain of rats with epilepsy.

Authors - H-Y Li
ratWB

Mol Biol Rep

Pramipexole attenuates 6-OHDA-induced Parkinson's disease by mediating the Nurr1/NF-κB pathway.

Authors - Hua Gao
humanWB

Exp Ther Med

Wnt1 silencing enhances neurotoxicity induced by paraquat and maneb in SH-SY5Y cells.

Authors - Cui Huang
mouseWB

Exp Ther Med

Attenuated macrophage activation mediated by microRNA-183 knockdown through targeting NR4A2.

Authors - Fu-Han Gong
humanWB

Cell Signal

miR-206 modulates lipopolysaccharide-mediated inflammatory cytokine production in human astrocytes.

Authors - Xiaodong Duan
humanWB,IHC

Cancer

Identification of low intratumoral gene expression heterogeneity in neuroblastic tumors by genome-wide expression analysis and game theory.

Authors - Albino Domenico D