NR4A3 Polyclonal antibody

NR4A3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55405-1-AP

Synonyms

TEC, NOR1, MINOR, CSMF, CHN

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Validation Data Gallery

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  WB analysis of mouse spleen using 55405-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 55405-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse thymus tissue, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

55405-1-AP targets NR4A3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nuclear receptor subfamily 4, group A, member 3
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: NM_006981
• Gene Symbol: NR4A3
• Gene ID (NCBI): 8013
• RRID: AB_2881322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptor 4A3 (NR4A3, also known as NOR-1), NR4A1 and NR4A2 are TFs belonging to NR4A orphan nuclear receptor subfamily. The role of all three NR4A members in regulating gene expression involved in cell proliferation, differentiation, apoptosis, inflammation and metabolism in cardiovascular diseases has been widely studied. In osteoblasts, NR4A receptor has been proved to regulate the expression and differentiation of osteoblast genes. It is reported that NR4A receptor is highly expressed in human coronary atherosclerotic lesions, and NR4A3 deficiency can reduce the proliferation and migration of VSMCs and the formation of neointima after vascular injury. Therefore, NR4A3 may play an important role in mediating phenotypic transformation of VSMC (PMID: 38629274). It is expected to be located in nucleus. The protein has three isforms, isoform alpha is highly expressed in skeletal muscle. Isoform beta is highly expressed in skeletal muscle and low expressed in fetal brain and placenta. The calculated molecular weight of NR4A3 is 68 kDa.

Publications

Species	Application	Title
rat	WB	Biology (Basel); CEBPA Overexpression Enhances β-Cell Proliferation and Survival; Authors - Peter N Ellsworth
human	WB,IHC	Aging (Albany NY); Oxidative stress-induced EGR1 upregulation promotes NR4A3-mediated nucleus pulposus cells apoptosis in intervertebral disc degeneration; Authors - Si-Kuan Zheng