Tested Applications
Positive WB detected in | HL-60 cells, Jurkat cells, THP-1 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
32308-1-AP targets NRAMP1 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide Predict reactive species |
Full Name | solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 |
Calculated Molecular Weight | 432 aa, 48 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC071165 |
Gene Symbol | SLC11A1 |
Gene ID (NCBI) | 6556 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | P49279 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC11A1 also known as NRAMP1, belongs to the SLC11 family of solute carriers, which mediate the transport of divalent metals. NRAMP1 is a divalent metal importer specifically expressed in the membranes of late endosomes/lysosomes of professional phagocytes. It plays a crucial role in the defense against intracellular pathogens by depleting essential metal ions from endocytosed microbes, thereby preventing their proliferation(PMID: 24832660, PMID: 39824808).
Protocols
Product Specific Protocols | |
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WB protocol for NRAMP1 antibody 32308-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |