NRAMP1 Polyclonal antibody

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• NRAMP1 Polyclonal antibody (32308-1-AP)

• 

  WB analysis using 32308-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32308-1-AP (NRAMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 32308-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32308-1-AP (NRAMP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

NRAMP1 Polyclonal Antibody for WB, ELISA

Cat No. 32308-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SLC11A1, NRAMP 1

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Tested Applications

• Positive WB detected in: HL-60 cells, Jurkat cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32308-1-AP targets NRAMP1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
• Calculated Molecular Weight: 432 aa, 48 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC071165
• Gene Symbol: SLC11A1
• Gene ID (NCBI): 6556
• RRID: AB_3742575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P49279
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC11A1 also known as NRAMP1, belongs to the SLC11 family of solute carriers, which mediate the transport of divalent metals. NRAMP1 is a divalent metal importer specifically expressed in the membranes of late endosomes/lysosomes of professional phagocytes. It plays a crucial role in the defense against intracellular pathogens by depleting essential metal ions from endocytosed microbes, thereby preventing their proliferation(PMID: 24832660, PMID: 39824808).