NRBP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NRBP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 21549-1-AP

Synonyms

NRBP2, pp9320, TRG 16, TRG16

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Validation Data Gallery

• 

  WB analysis of mouse kidney using 21549-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21549-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21549-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21549-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 21549-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21549-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 21549-1-AP

  IP result of anti-NRBP2 (IP:21549-1-AP, 4ug; Detection:21549-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human kidney using 21549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21549-1-AP (NRBP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 21549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21549-1-AP (NRBP2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human testis tissue, human placenta tissue, mouse brain tissue, HeLa cells, A549 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 21549-1-AP is NRBP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NRBP2 fusion protein Ag16215
• Full Name: nuclear receptor binding protein 2
• Calculated Molecular Weight: 501 aa, 58 kDa
• Observed Molecular Weight: 33 kDa, 58 kDa
• GenBank Accession Number: BC113873
• Gene Symbol: NRBP2
• Gene ID (NCBI): 340371
• RRID: AB_10733219
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.

Publications

Species	Application	Title
human	WB,IHC	Front Oncol; NRBP2 Functions as a Tumor Suppressor and Inhibits Epithelial-to-Mesenchymal Transition in Breast Cancer.; Authors - Zhiyu Li ; KD Validated
human	WB	Ann Transl Med; PSMD12 promotes glioma progression by upregulating the expression of Nrf2.; Authors - Zhongyong Wang
human,mouse	WB,IHC	Mol Cell Biochem; TRG16, targeted by miR-765, inhibits breast cancer stem cell-like properties via regulating the NF-κB pathway.; Authors - Feng Chi ; KD Validated
human	WB	Cancers (Basel); Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression.; Authors - Anqi Xiong

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