NRBP2 Polyclonal antibody

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• KD/KO Validated

NRBP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 21549-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

NRBP2, pp9320, TRG 16, TRG16

• 

  WB analysis of mouse kidney using 21549-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human testis using 21549-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 21549-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 21549-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 21549-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 21549-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 21549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse kidney using 21549-1-AP

  IP result of anti-NRBP2 (IP:21549-1-AP, 4ug; Detection:21549-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 21549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21549-1-AP (NRBP2 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 21549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21549-1-AP (NRBP2 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human testis tissue, human placenta tissue, mouse brain tissue, HeLa cells, A549 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21549-1-AP targets NRBP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16215

  Product name: Recombinant human NRBP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 138-258 aa of BC113873

  Sequence: PLMNFAATRPLGLPRVLAPPPEEVQKAKTPTPEPFDSETRKVIQMQCNLERSEDKARWHLTLLLVLEDRLHRQLTYDLLPTDSAQDLASELVHYGFLHEDDRMKLAAFLESTFLKYRGTQA

• Full Name: nuclear receptor binding protein 2
• Calculated Molecular Weight: 501 aa, 58 kDa
• Observed Molecular Weight: 33 kDa, 58 kDa
• GenBank Accession Number: BC113873
• Gene Symbol: NRBP2
• Gene ID (NCBI): 340371
• RRID: AB_10733219
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NSY0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.

Publications

What published studies show

The NRBP2 Polyclonal antibody (Cat# 21549-1-AP) has a total of 5 citations appearing in Nature Communications, Cancers (Basel), Molecular and Cellular Biochemistry, Frontiers in Oncology, and Annals of Translational Medicine. The associated research fields span L1 retrotransposition regulation, medulloblastoma tumor biology, breast cancer stem cell properties and epithelial-to-mesenchymal transition, and glioma progression.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Nat Commun; Opposing roles of pseudokinases NRBP1 and NRBP2 in regulating L1 retrotransposition; Authors - Wei Yang
human	WB	Cancers (Basel); Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression.; Authors - Anqi Xiong
human,mouse	WB,IHC	Mol Cell Biochem; TRG16, targeted by miR-765, inhibits breast cancer stem cell-like properties via regulating the NF-κB pathway.; Authors - Feng Chi ; KD Validated
human	WB,IHC	Front Oncol; NRBP2 Functions as a Tumor Suppressor and Inhibits Epithelial-to-Mesenchymal Transition in Breast Cancer.; Authors - Zhiyu Li ; KD Validated
human	WB	Ann Transl Med; PSMD12 promotes glioma progression by upregulating the expression of Nrf2.; Authors - Zhongyong Wang