NRF1/nuclear respiratory factor 1 Recombinant monoclonal antibody

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• NRF1/nuclear respiratory factor 1 Recombinant monoclonal antibody (83092-1-RR)

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• KD/KO Validated

NRF1/nuclear respiratory factor 1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Cat No. 83092-1-RR

Clone No.230394B1

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Synonyms

NRF1, nuclear respiratory factor 1, 230394B1, ALPHA PAL, Alpha palindromic-binding protein

• 

  WB analysis using 83092-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83092-1-RR (NRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 83092-1-RR

  WB result of NRF1/nuclear respiratory factor 1 antibody (83092-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NRF1/nuclear respiratory factor 1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical squamous cancer using 83092-1-RR

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83092-1-RR (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 83092-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRF1/nuclear respiratory factor 1 antibody (83092-1-RR, Clone: 230394B1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HEK-293T using 83092-1-RR

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human NRF1 (83092-1-RR, Clone:230394B1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  ChIP experiment of HeLa using 83092-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of NRF1/nuclear respiratory factor 1 (83092-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 83092-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83092-1-RR against Human NRF1/nuclear respiratory factor 1 were performed. The affinity constant is 0.115 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, RAW 264.7 cells, NIH/3T3 cells, U2OS cells
• Positive IHC detected in: human cervical squamous cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HEK-293T cells
• Positive ChIP-qPCR detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83092-1-RR targets NRF1/nuclear respiratory factor 1 in WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3172

  Product name: Recombinant human NRF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 213-522 aa of BC016925

  Sequence: LKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLFMADRAGRKWILTDKATGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ

• Full Name: nuclear respiratory factor 1
• Calculated Molecular Weight: 522 aa, 56 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC016925
• Gene Symbol: NRF1
• Gene ID (NCBI): 4899
• RRID: AB_3670806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16656
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
chicken	WB,IF	Genes (Basel); PPP1CC Suppresses Preadipocyte Differentiation in Chickens at Least Partly by Regulating NRF1 Expression.; Authors - Tingting Cui
mouse	IHC	Front Endocrinol (Lausanne); Effects of different sugar-lipid ratio diets on the occurrence of type 2 diabetes mellitus.; Authors - Wenjie Sun ; KD Validated