GABPA Polyclonal antibody

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• GABPA Polyclonal antibody (21542-1-AP)

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GABPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21542-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

NFT2, GABP subunit alpha, GA-binding protein alpha chain, GA binding protein alpha chain, E4TF1A

• 

  WB analysis using 21542-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 21542-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 21542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 21542-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 21542-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 21542-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21542-1-AP (GABPA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 21542-1-AP

  IP result of anti-GABPA (IP:21542-1-AP, 4ug; Detection:21542-1-AP 1:1000) with HeLa cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 21542-1-AP

  IP result of anti-GABPA (IP:21542-1-AP, 4ug; Detection:21542-1-AP 1:1000) with HeLa cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21542-1-AP (GABPA antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21542-1-AP (GABPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21542-1-AP (GABPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 21542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21542-1-AP (GABPA antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 21542-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GABPA antibody (21542-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, mouse liver tissue, MCF-7 cells, A549 cells, rat brain tissue, K-562 cells, NIH/3T3 cells, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21542-1-AP targets GABPA in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16191

  Product name: Recombinant human GABPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-321 aa of BC035031

  Sequence: MTKREAEELIEIEIDGTEKAECTEESIVEQTYAPAECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLDPERSLFDQGVKTDGTVQLSVQVISYQGIEPKLNILEIVKPADTVEVVIDPDAHHAESEAHLVEEAQVITLDGTKHITTISDETSEQVTRWAAALEGYRKEQERLGIPYDPIQWSTDQVLHWVVWVMKEFSMTDIDLTTLNISGRELCSLNQEDFFQRVPRGEILWSHLELLRKYVLASQEQQMNEIVTIDQPVQIIPASVQSATPTTIKVINSSAKAAKVQRAPRISGEDRSSPGNRTGNNGQIQ

• Full Name: GA binding protein transcription factor, alpha subunit 60kDa
• Calculated Molecular Weight: 454 aa, 51 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC035031
• Gene Symbol: GABPA
• Gene ID (NCBI): 2551
• RRID: AB_10858481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q06546
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Stem Cell; PSPC1 exerts an oncogenic role in AML by regulating a leukemic transcription program in cooperation with PU.1; Authors - Juyeong Hong
	WB,IF	Nat Cell Biol; The transcription factor GABPA is a master regulator of naive pluripotency; Authors - Chengjie Zhou
		Sci Bull (Beijing); GABPA recruits the Integrator endonuclease complex to promote transcription elongation.; Authors - Hang Yang
		Hepatology; High mobility group protein B1 controls liver cancer initiation through yes-associated protein -dependent aerobic glycolysis.; Authors - Ruochan Chen
human	WB	Redox Biol; Nrf1 is an indispensable redox-determining factor for mitochondrial homeostasis by integrating multi-hierarchical regulatory networks; Authors - Shaofan Hu
human	ChIP	Mol Cell; The immortality mechanism of TERT promoter mutant cancers is self-reinforcing and reversible; Authors - Nicholas O Stevers