Tested Applications
| Positive WB detected in | MCF-7 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse stomach tissue, human stomach tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10527-1-AP targets NRG1, isoform SMDF in WB, IHC, IF/ICC, IF-P, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0803 Product name: Recombinant human NRG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-225 aa of BC007675 Sequence: MEIYSPDMSEVAAERSSSPSTQLSADPSLDGLPAAEDMPEPQTEDGRTPGLVGLAVPCCACLEAERLRGCLNSEKICIVPILACLVSLCLCIAGLKWVFVDKIFEYDSPTHLDPGGLGQDPIISLDATAASALWVSSEAYTSPVSRAQSESEVQVTVQGDKAVVSFEPSAAPTPKNRIFAFSFLPSTAPSFPSPTRNPEVRTPKSATQPQTTETNLQTAPKLSTS Predict reactive species |
| Full Name | neuregulin 1 |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC007675 |
| Gene Symbol | NRG1 |
| Gene ID (NCBI) | 3084 |
| RRID | AB_2154659 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02297 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. Studies has been reportrd, the full-length type III NRG1 (NRG1 isoform SMDF) is more than 110 kDa, the 70-75 kDa band is a membrane bound form and it can be cleaved by an unknown enzyme releases the ∼45 kDa secreted form of NRG1(PMID: 21047912, PMID: 11425901). The immunogen of this antibody is against NRG1 isoform SMDF.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NRG1, isoform SMDF antibody 10527-1-AP | Download protocol |
| IHC protocol for NRG1, isoform SMDF antibody 10527-1-AP | Download protocol |
| WB protocol for NRG1, isoform SMDF antibody 10527-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
NRG1, isoform SMDF Polyclonal antibody (Cat# 10527-1-AP) has 28 citations across 24 journals, including high-impact publications in Cancer Cell, Cell, Cell Metabolism, Advanced Science, Frontiers in Immunology, International Journal of Oncology, Liver International, and International Immunopharmacology. Associated research fields span oncology (bladder, liver, breast, thyroid cancers), neuroscience (brain development, neuroinflammation, spinal cord injury), cardiology (cardiac injury, heart failure), immunology (macrophage polarization), fibrosis, and metabolic diseases.
| Species | Application | Title |
|---|---|---|
Cancer Cell Interferon-dependent SLC14A1(+) cancer-associated fibroblasts promote cancer stemness via WNT5A in bladder cancer | ||
Cell Spatiotemporal transcriptome atlas reveals the regional specification of the developing human brain | ||
Cell Metab Liver-breast communication of adipocyte-oriented exosomes drives primary mammary cancer progression | ||
J Exp Clin Cancer Res miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling. | ||
Adv Sci (Weinh) HLA-DR(+) Schwann Cells Generate the Protumor Cancer-Neuron-Immune Niche in Head and Neck Squamous Cell Carcinoma. | ||
Adv Healthc Mater Phyhip-Targeted Delivery of bFGF Platform with Dual Modulation for Enhancing Neurovascular Repair in Traumatic Brain Injury. |
