NRG1, isoform SMDF Polyclonal antibody
NRG1, isoform SMDF Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10527-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human liver tissue, mouse liver tissue, SH-SY5Y cells |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | mouse stomach tissue, human stomach tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 6 publications below |
IHC | See 3 publications below |
IF | See 3 publications below |
Product Information
10527-1-AP targets NRG1, isoform SMDF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NRG1, isoform SMDF fusion protein Ag0803 |
Full Name | neuregulin 1 |
Calculated molecular weight | 32 kDa |
Observed molecular weight | 55 kDa and 72 kDa |
GenBank accession number | BC007675 |
Gene symbol | NRG1 |
Gene ID (NCBI) | 3084 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.
Protocols
Product Specific Protocols | |
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WB protocol for NRG1, isoform SMDF antibody 10527-1-AP | Download protocol |
IHC protocol for NRG1, isoform SMDF antibody 10527-1-AP | Download protocol |
IF protocol for NRG1, isoform SMDF antibody 10527-1-AP | Download protocol |
IP protocol for NRG1, isoform SMDF antibody 10527-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
ACS Biomater Sci Eng Sericin Nerve Guidance Conduit Delivering Therapeutically Repurposed Clobetasol for Functional and Structural Regeneration of Transected Peripheral Nerves | ||
Ecotoxicol Environ Saf Prenatal di-(2-ethylhexyl) phthalate exposure induced myocardial cytotoxicity via the regulation of the NRG1-dependent ErbB2/ErbB4-PI3K/AKT signaling pathway in fetal mice. | ||
J Exp Clin Cancer Res miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling. | ||
Am J Physiol Renal Physiol ErbB4 deletion accelerates renal fibrosis following renal injury. | ||
Folia Neuropathol miR-143-3p inhibition promotes neuronal survival in an Alzheimer's disease cell model by targeting neuregulin-1. | ||
Evid Based Complement Alternat Med Extracellular Vesicle-Encapsulated miR-183-5p from Rhynchophylline-Treated H9c2 Cells Protect against Methamphetamine-Induced Dependence in Mouse Brain by Targeting NRG1. |