NRG1, isoform SMDF Polyclonal antibody

NRG1, isoform SMDF Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 10527-1-AP


ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRGA, NDF, Neu differentiation factor, neuregulin 1, Neuregulin 1 isoform SMDF, NRG1, NRG1 isoform SMDF, NRG1, isoform SMDF, Pro NRG1, SMDF

Tested Applications

Positive WB detected inmouse liver tissue, SH-SY5Y cells
Positive IHC detected inmouse stomach tissue, human stomach tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10527-1-AP targets NRG1, isoform SMDF in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRG1, isoform SMDF fusion protein Ag0803
Full Name neuregulin 1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007675
Gene Symbol NRG1
Gene ID (NCBI) 3084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. Studies has been reportrd, the full-length type III NRG1 (NRG1 isoform SMDF) is more than 110 kDa, the 70-75 kDa band is a membrane bound form and it can be cleaved by an unknown enzyme releases the ∼45 kDa secreted form of NRG1(PMID: 21047912, PMID: 11425901). The antiiody is specific to NRG1 isoform SMDF.


Product Specific Protocols
WB protocol for NRG1, isoform SMDF antibody 10527-1-APDownload protocol
IHC protocol for NRG1, isoform SMDF antibody 10527-1-APDownload protocol
IF protocol for NRG1, isoform SMDF antibody 10527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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