Validation Data Gallery
|Positive WB detected in||human liver tissue, mouse liver tissue, SH-SY5Y cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||mouse stomach tissue, human stomach tissue, mouse cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10527-1-AP targets NRG1, isoform SMDF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NRG1, isoform SMDF fusion protein Ag0803|
|Full Name||neuregulin 1|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||55 kDa and 72 kDa|
|GenBank accession number||BC007675|
|Gene ID (NCBI)||3084|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.
|Product Specific Protocols|
|WB protocol for NRG1, isoform SMDF antibody 10527-1-AP||Download protocol|
|IHC protocol for NRG1, isoform SMDF antibody 10527-1-AP||Download protocol|
|IF protocol for NRG1, isoform SMDF antibody 10527-1-AP||Download protocol|
|IP protocol for NRG1, isoform SMDF antibody 10527-1-AP||Download protocol|
|Click here to view our Standard Protocols|
ACS Biomater Sci Eng
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Ecotoxicol Environ Saf
Prenatal di-(2-ethylhexyl) phthalate exposure induced myocardial cytotoxicity via the regulation of the NRG1-dependent ErbB2/ErbB4-PI3K/AKT signaling pathway in fetal mice.
J Exp Clin Cancer Res
miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling.
Am J Physiol Renal Physiol
ErbB4 deletion accelerates renal fibrosis following renal injury.
miR-143-3p inhibition promotes neuronal survival in an Alzheimer's disease cell model by targeting neuregulin-1.
Evid Based Complement Alternat Med
Extracellular Vesicle-Encapsulated miR-183-5p from Rhynchophylline-Treated H9c2 Cells Protect against Methamphetamine-Induced Dependence in Mouse Brain by Targeting NRG1.