|Positive WB detected in||human liver tissue, mouse liver tissue, SH-SY5Y cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||mouse stomach tissue, human stomach tissue, mouse cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10527-1-AP targets NRG1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NRG1 fusion protein Ag0803|
|Full Name||neuregulin 1|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||55 kDa and 72 kDa|
|GenBank accession number||BC007675|
|Gene ID (NCBI)||3084|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain.
|Product Specific Protocols|
|WB protocol for NRG1 antibody 10527-1-AP||Download protocol|
|IHC protocol for NRG1 antibody 10527-1-AP||Download protocol|
|IF protocol for NRG1 antibody 10527-1-AP||Download protocol|
|IP protocol for NRG1 antibody 10527-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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