NRIP3 Polyclonal antibody

NRIP3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15664-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

C11orf14, NRIP3, NY SAR 105, Sarcoma antigen NY SAR 105

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of MCF-7 using 15664-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 15664-1-AP (NRIP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 15664-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15664-1-AP (NRIP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse ovary using 15664-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 15664-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 15664-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse ovary using 15664-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	MCF-7 cells
Positive IHC detected in	mouse ovary tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15664-1-AP targets NRIP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8097 Product name: Recombinant human NRIP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-241 aa of BC008835 Sequence: MFYSGLLTEGGRKETDMREAASLRQQRRMKQAVQFIHKDSADLLPLDGLKKLGSSKDMQPHNILQRRLMETNLSKLRSGPRVPWASKTNKLNQAKSEGLKKSEEDDMILVSCQCAGKDVKALVDTGCLYNLISLACVDRLGLKEHVKSHKHEGEKLSLPRHLKVVGQIEHLVITLGSLRLDCPAAVVDDNEKNLSLGLQTLRSLKCIINLDKHRLIMGKTDKEEIPFVETVSLNEDNTSEA Predict reactive species
Full Name	nuclear receptor interacting protein 3
Calculated Molecular Weight	241 aa, 27 kDa
Observed Molecular Weight	27 kDa
GenBank Accession Number	BC008835
Gene Symbol	NRIP3
Gene ID (NCBI)	56675
RRID	AB_2878165
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NQ35
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for NRIP3 antibody 15664-1-AP	Download protocol
WB protocol for NRIP3 antibody 15664-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The NRIP3 Polyclonal antibody (Cat# 15664-1-AP) has 1 total citation. The cited publication appeared in Research Square (Res Sq) in 2025, using IHC on human samples. The research field is neurodegenerative disease, specifically investigating pathological mechanisms in the hippocampus across aged control, primary age-related tauopathy, and Alzheimer's disease subjects via spatial transcriptomic analysis.

Species	Application	Title
human	IHC	Res Sq Novel Pathological Mechanisms Revealed by Spatial Transcriptomic Analysis of Hippocampus in Aged Control, Primary Age-Related Tauopathy, and Alzheimer's Disease. Authors - Hong-Wen Deng View Article