Tested Applications
| Positive IHC detected in | human retinoblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17388-1-AP targets NRL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11277 Product name: Recombinant human NRL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC012395 Sequence: MALPPSPLAMEYVNDFDLMKFEVKREPSEGRPGPPTASLGSTPYSSVPPSPTFSEPGMVGATEGTRPGLEELYWLATLQQQLGAGEALGLSPEEAMELLQGQGPVPVDGPHGYYPGSPEETGAQHVQLAERFSDAALVSMSVRELNRQLRGCGRDEALRLKQRRRTLKNRGYAQACRSKRLQQRRGLEAERARLAAQLDALRAEVARLARERDLYKARCDRLTSSGPGSGDPSHLFL Predict reactive species |
| Full Name | neural retina leucine zipper |
| Calculated Molecular Weight | 237 aa, 26 kDa |
| GenBank Accession Number | BC012395 |
| Gene Symbol | NRL |
| Gene ID (NCBI) | 4901 |
| RRID | AB_2878400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54845 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NRL antibody 17388-1-AP | Download protocol |
| WB protocol for NRL antibody 17388-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NRL Polyclonal antibody (Cat# 17388-1-AP) has 3 citations published in Cell Stem Cell, Nature Protocols, and JCI Insight. The cited research spans brain organoid development (functionally integrated bilateral optic vesicles), iPSC-derived forebrain organoid generation with eye primordia assembly, and retinal degeneration rescue via Nrl gene inactivation in Pde6a mice. Applications used include immunofluorescence (IF) and Western blot (WB) in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Human brain organoids assemble functionally integrated bilateral optic vesicles. | ||
Nat Protoc Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia | ||
JCI Insight All-in-one AAV-mediated Nrl gene inactivation rescues retinal degeneration in Pde6a mice |
Reviews
What customers say
Both reviewers gave this anti-NRL antibody a 5-star rating. One user confirmed reliable Western Blot performance in P30 mouse retina at a 1:1000 dilution. The other reported excellent Immunofluorescence results with no unspecific staining. Overall, the antibody is well-regarded for its specificity and consistent performance across applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zhiquan (Verified Customer) (01-12-2024) | This anti-NRL antibody works well for WB in P30 Mouse retina.
 |
Alessandro (Verified Customer) (01-19-2023) | great outcome, no unspecific staining
|
