neurturin-Specific Polyclonal antibody

neurturin-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19709-1-AP

Synonyms

neurturin, neurturin-Specific, NRTN, NTN

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Validation Data Gallery

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  WB analysis of human liver using 19709-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 19709-1-AP (neurturin-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 19709-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 19709-1-AP (neurturin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 19709-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19709-1-AP (neurturin-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19709-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19709-1-AP (neurturin-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19709-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19709-1-AP (neurturin-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human liver tissue, human heart tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19709-1-AP targets neurturin-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: neurturin
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_004558
• Gene Symbol: NRTN
• Gene ID (NCBI): 4902
• RRID: AB_10644284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRTN belongs to the TGF-beta family and GDNF subfamily. NRTN supports the survival of sympathetic neurons in culture. NRTN may regulate the development and maintenance of the CNS. It might control the size of non-neuronal cell population such as haemopoietic cells. Defects in NRTN are a cause of Hirschsprung disease (HSCR). The antibody is specific to NRTN.