NSF Polyclonal antibody

NSF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21172-1-AP

Synonyms

NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF

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Validation Data Gallery

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  WB analysis using 21172-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21172-1-AP (NSF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21172-1-AP

  IP result of anti-NSF (IP:21172-1-AP, 4ug; Detection:21172-1-AP 1:300) with mouse brain tissue lysate 3000ug.

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  IHC staining of mouse brain using 21172-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21172-1-AP (NSF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of mouse brain using 21172-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21172-1-AP (NSF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

21172-1-AP targets NSF in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSF fusion protein Ag15422
• Full Name: N-ethylmaleimide-sensitive factor
• Calculated Molecular Weight: 744 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC030613
• Gene Symbol: NSF
• Gene ID (NCBI): 4905
• RRID: AB_2878823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The N-ethylmaleimide-sensitive fusion protein (NSF) is a 70-83 kDa cytosol protein that is expressed in all cell types. NSF functions as an ATPase and it is a part of the 20S membrane fusion apparatus with the vesicular SNAP receptor (v-SNARE) synaptobrevin, and the target SNAREs (t-SNARE) syntaxin and SNAP-25. With the hydrolysis of ATP by NSF, the released energy may set-up or drive membrane fusion processes. NSF is required for general membrane transport and trafficking.

Publications

Species	Application	Title
mouse	IF	Cell Death Differ; Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy.; Authors - Zhaokang Cui