• Featured Product
  • KD/KO Validated

NSUN2 Polyclonal antibody

NSUN2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20854-1-AP

Synonyms

hTrm4, MISU, NSUN2, SAKI, TRM4



Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20854-1-AP targets NSUN2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSUN2 fusion protein Ag14791
Full Name NOL1/NOP2/Sun domain family, member 2
Calculated Molecular Weight 767 aa, 86 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
RRIDAB_10693629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

Protocols

Product Specific Protocols
WB protocol for NSUN2 antibody 20854-1-APDownload protocol
IHC protocol for NSUN2 antibody 20854-1-APDownload protocol
IF protocol for NSUN2 antibody 20854-1-APDownload protocol
IP protocol for NSUN2 antibody 20854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Signal Transduct Target Ther

tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis

Authors - Sunyang Ying
humanWB

Nat Biotechnol

Identification of direct targets and modified bases of RNA cytosine methyltransferases.

Authors - Khoddami Vahid V
mouseWB

Cell

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Authors - Deniz Simsek
humanWB,IHC

Nat Cell Biol

5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.

Authors - Xin Chen
humanWB

Nat Protoc

Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.

Authors - Vahid Khoddami
humanWB,IF

Cell Host Microbe

Epitranscriptomic Addition of m5C to HIV-1 Transcripts Regulates Viral Gene Expression.

Authors - David G Courtney
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sarah (Verified Customer) (01-05-2021)

Works perfectly for WB. Tested on HeLa cells and HEK293T.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa and HEL293T cells