|Positive WB detected in||HEK-293, COLO 320 cells, HeLa cells, HepG2 cells, MCF-7 cells, SH-SY5Y cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20854-1-AP is NSUN2 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NSUN2 fusion protein Ag14791|
|Full Name||NOL1/NOP2/Sun domain family, member 2|
|Calculated molecular weight||767 aa, 86 kDa|
|Observed molecular weight||90-100 kDa|
|GenBank accession number||BC001041|
|Gene ID (NCBI)||54888|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.
|Product Specific Protocols|
|WB protocol for NSUN2 antibody 20854-1-AP||Download protocol|
|IHC protocol for NSUN2 antibody 20854-1-AP||Download protocol|
|IF protocol for NSUN2 antibody 20854-1-AP||Download protocol|
|IP protocol for NSUN2 antibody 20854-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nat Struct Mol Biol
Genome-wide identification of mRNA 5-methylcytosine in mammals.
Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.
Cytosine-5 RNA methylation links protein synthesis to cell metabolism.
Genomics Proteomics Bioinformatics
Epitranscriptomic 5-Methylcytosine Profile in PM2.5-induced Mouse Pulmonary Fibrosis.
Front Cell Dev Biol
Disease Activity-Associated Alteration of mRNA m5 C Methylation in CD4+ T Cells of Systemic Lupus Erythematosus.
Exp Cell Res
RNA methyltransferase NSUN2 promotes hypopharyngeal squamous cell carcinoma proliferation and migration by enhancing TEAD1 expression in an m5C-dependent manner.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sarah (Verified Customer) (01-05-2021)
Works perfectly for WB. Tested on HeLa cells and HEK293T.