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  • KD/KO Validated

NSUN2 Polyclonal antibody

NSUN2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20854-1-AP

Synonyms

hTrm4, MISU, NSUN2, SAKI, TRM4



Tested Applications

Positive WB detected inHEK-293, COLO 320 cells, HeLa cells, HepG2 cells, MCF-7 cells, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20854-1-AP is NSUN2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSUN2 fusion protein Ag14791
Full Name NOL1/NOP2/Sun domain family, member 2
Calculated molecular weight 767 aa, 86 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC001041
Gene symbol NSUN2
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

Protocols

Product Specific Protocols
WB protocol for NSUN2 antibody 20854-1-APDownload protocol
IHC protocol for NSUN2 antibody 20854-1-APDownload protocol
IF protocol for NSUN2 antibody 20854-1-APDownload protocol
IP protocol for NSUN2 antibody 20854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

Genome-wide identification of mRNA 5-methylcytosine in mammals.

Authors - Tao Huang
  • KD Validated
humanWB

Nat Protoc

Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.

Authors - Vahid Khoddami
humanWB

PLoS Biol

Cytosine-5 RNA methylation links protein synthesis to cell metabolism.

Authors - Nikoletta A Gkatza
  • KO Validated
mouseWB

Genomics Proteomics Bioinformatics

Epitranscriptomic 5-Methylcytosine Profile in PM2.5-induced Mouse Pulmonary Fibrosis.

Authors - Xiao Han
humanWB

Front Cell Dev Biol

Disease Activity-Associated Alteration of mRNA m5 C Methylation in CD4+ T Cells of Systemic Lupus Erythematosus.

Authors - Gangqiang Guo
humanWB

Exp Cell Res

RNA methyltransferase NSUN2 promotes hypopharyngeal squamous cell carcinoma proliferation and migration by enhancing TEAD1 expression in an m5C-dependent manner.

Authors - Lixiao Chen
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sarah (Verified Customer) (01-05-2021)

Works perfectly for WB. Tested on HeLa cells and HEK293T.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa and HEL293T cells