NSUN4 Polyclonal antibody, PBS Only

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• NSUN4 Polyclonal antibody, PBS Only (29786-1-PBS)

• 

  WB analysis using 29786-1-AP (same clone as 29786-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29786-1-AP (NSUN4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29786-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 29786-1-AP (same clone as 29786-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29786-1-AP (NSUN4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29786-1-PBS in a different storage buffer formulation.

NSUN4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29786-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4

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Product Information

29786-1-PBS targets NSUN4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31821

  Product name: Recombinant human NSUN4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 26-284 aa of BC014441

  Sequence: RYKKKWAATEPKFPAVRLALQNFDMAYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQIL

• Full Name: NOL1/NOP2/Sun domain family, member 4
• Calculated Molecular Weight: 384 aa, 43 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC014441
• Gene Symbol: NSUN4
• Gene ID (NCBI): 387338
• RRID: AB_2935480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96CB9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly. The calculated MW of NSUN4 is 43 kDa, 29786-1-AP can detect the band around 40 kDa.(PMID:22949673, 24516400)