Validation Data Gallery
|Positive WB detected in||HeLa cells, A549 cells, human kidney tissue, human placenta tissue|
|Positive IHC detected in||mouse skeletal muscle tissue, human placenta tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15449-1-AP targets NSUN5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NSUN5 fusion protein Ag7711|
|Full Name||NOL1/NOP2/Sun domain family, member 5|
|Calculated molecular weight||46.6 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC008084|
|Gene ID (NCBI)||55695|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Nat Chem Biol
Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.
Cognitive deficits in mice lacking Nsun5, a cytosine-5 RNA methyltransferase, with impairment of oligodendrocyte precursor cells.
Agenesis and Hypomyelination of Corpus Callosum in Mice Lacking Nsun5, an RNA Methyltransferase.
Identification of Expression Patterns and Potential Prognostic Significance of m5C-Related Regulators in Head and Neck Squamous Cell Carcinoma.
Expression of the RNA methyltransferase Nsun5 is essential for developing cerebral cortex.
Cell Biochem Biophys
NSUN5-FTH1 Axis Inhibits Ferroptosis to Promote the Growth of Gastric Cancer Cells