• Featured Product
  • KD/KO Validated

NSUN6 Polyclonal antibody

NSUN6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17240-1-AP


4933414E04Rik, NOPD1, NSUN6

Tested Applications

Positive WB detected inNeuro-2a cells, HEK-293 cells, HepG2 cells, mouse brain
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17240-1-AP targets NSUN6 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSUN6 fusion protein Ag11084
Full Name NOL1/NOP2/Sun domain family, member 6
Calculated Molecular Weight 469 aa, 52 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC035778
Gene Symbol NSUN6
Gene ID (NCBI) 221078
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSUN6 is an RNA methyltransferase that catalyzes the methylation at position cytosine C72 in some tRNAs. NSUN6 has been reported to localize to the cytoplasm and largely colocalize with Golgi apparatus and pericentriolar matrix, indicating that NSUN6 modifies tRNAs in a late step in their biogenesis. NSUN6 may also play a major role in bone metastasis and could be a valuable therapeutic target for bone metastasis.


Product Specific Protocols
WB protocol for NSUN6 antibody 17240-1-APDownload protocol
IHC protocol for NSUN6 antibody 17240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nucleic Acids Res

Sequence- and structure-specific cytosine-5 mRNA methylation by NSUN6.

Authors - Tommaso Selmi
  • KO Validated

PLoS Biol

Cytosine-5 RNA methylation links protein synthesis to cell metabolism.

Authors - Nikoletta A Gkatza

Mol Syst Biol

CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators.

Authors - Liang Fang
  • KD Validated

Nat Struct Mol Biol

Genome-wide identification of mRNA 5-methylcytosine in mammals.

Authors - Tao Huang
  • KD Validated

Exp Ther Med

Loss of NSUN6 inhibits osteosarcoma progression by downregulating EEF1A2 expression and activation of Akt/mTOR signaling pathway via m5C methylation

Authors - Sang Hu
  • KD Validated

J Cell Mol Med

RNA m5C methylation orchestrates BLCA progression via macrophage reprogramming

Authors - Dali Yan
  • KO Validated