NSUN7 Polyclonal antibody, PBS Only

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• NSUN7 Polyclonal antibody, PBS Only (17546-1-PBS)

• 

  WB analysis using 17546-1-AP (same clone as 17546-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17546-1-AP (NSUN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17546-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17546-1-AP (same clone as 17546-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17546-1-AP (NSUN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17546-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 17546-1-AP (same clone as 17546-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17546-1-AP (NSUN7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17546-1-PBS in a different storage buffer formulation.

NSUN7 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 17546-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

FLJ14001

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Product Information

17546-1-PBS targets NSUN7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11650

  Product name: Recombinant human NSUN7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 125-475 aa of BC036568

  Sequence: SLIIVMLYDFQDRKFQTRVLSDNEEPISEVQEVENLLNSFKIKLAAALARCRIKHDALSIYHILPETVRKQELRASTLPLYAWINTCKISPEEVYNNLKRRGYNKVKSVLHIDDKVFAVDQHCYDVLIFPSHLKNDLINIDLFKDYKLIFQDKSRSLAVHSVKALLNMDDDVLMVNTGSWYTVAHMSILTNNNTSKVFVCGVQSQAKDPDLKTLFTKIGCKNIEILHEKFINIESKDHRLQKVKVILLLPRCSGLGVSNPVEFILNEHEDTEFLKDHSQGGISVDKLHVLAQQQYEQLTHAMKFTKAQAVVYCTCSVFPEENEAVVKKALEFQDLGNKGQPYSGTLLRQCL

• Full Name: NOL1/NOP2/Sun domain family, member 7
• Calculated Molecular Weight: 718 aa, 81 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC036568
• Gene Symbol: NSUN7
• Gene ID (NCBI): 79730
• RRID: AB_2878415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NE18
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163).