NT5C Polyclonal antibody

NT5C Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 16577-1-AP

Synonyms

5', 3' nucleotidase, cytosolic, cdN, Deoxy 5 nucleotidase 1, DNT, dNT 1, DNT1, NT5C, P5N2, PN I, PN II, UMPH2

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Validation Data Gallery

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  IHC staining of mouse skeletal muscle using 16577-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16577-1-AP (NT5C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 16577-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16577-1-AP (NT5C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 16577-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16577-1-AP (NT5C Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 16577-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16577-1-AP (NT5C Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse skeletal muscle tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16577-1-AP targets NT5C in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NT5C fusion protein Ag9862
• Full Name: 5', 3'-nucleotidase, cytosolic
• Calculated Molecular Weight: 201 aa, 23 kDa
• GenBank Accession Number: BC017454
• Gene Symbol: NT5C
• Gene ID (NCBI): 30833
• RRID: AB_2153940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NT5C, also named as DNT1 and UMPH2, has two isoforms with calculated MW 23 kDa and 13 kDa.