NT5C3L Polyclonal antibody

NT5C3L Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16292-1-AP

Synonyms

NT5C3B, NT5C3L

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Validation Data Gallery

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  WB analysis of mouse stomach using 16292-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 16292-1-AP (NT5C3L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16292-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16292-1-AP (NT5C3L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16292-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16292-1-AP (NT5C3L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 16292-1-AP

  IP result of anti-NT5C3L (IP:16292-1-AP, 4ug; Detection:16292-1-AP 1:300) with mouse testis tissue lysate 4000 ug.

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Tested Applications

• Positive WB detected in: mouse stomach tissue, human testis tissue, mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16292-1-AP targets NT5C3L in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NT5C3L fusion protein Ag9458
• Full Name: 5'-nucleotidase, cytosolic III-like
• Calculated Molecular Weight: 292 aa, 34 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC014132
• Gene Symbol: NT5C3L
• Gene ID (NCBI): 115024
• RRID: AB_2251490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.