NT5DC1 Polyclonal antibody

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• NT5DC1 Polyclonal antibody (24102-1-AP)

NT5DC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24102-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

NT5C2L1, EC:3.1.3.-, 5'-nucleotidase domain-containing protein 1

• 

  WB analysis of MCF-7 using 24102-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 24102-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 24102-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 24102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 24102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human thyroid cancer using 24102-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human thyroid cancer using 24102-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 24102-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NT5DC1 antibody (24102-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF7 cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human lung cancer tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24102-1-AP targets NT5DC1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20916

  Product name: Recombinant human NT5DC1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-297 aa of BC015138

  Sequence: WKDIVAAIQHNYKMSAFKENCGIYFPEIKRDPGRYLHSCPESVKKWLRQLKNAGKILLLITSSHSDYCRLLCEYILGNDFTDLFDIVITNALKPGFFSHLPSQRPFRTLENDEEQEALPSLDKPGWYSQGNAVHLYELLKKMTGKPEPKVVYFGDSMHSDIFPARHYSNWETVLILEELRGDEGTRSQRPEESEPLEKKGKYEGPKAKPLNTSSKKWGSFFIDSVLGLENTEDSLVYTWSCKRISTYSTIAIPSIEAIAELPLDYKFTRFSSSNSKTAGYYPNPPLVLSSDETLISK

• Full Name: 5'-nucleotidase domain containing 1
• Calculated Molecular Weight: 455 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC015138
• Gene Symbol: NT5DC1
• Gene ID (NCBI): 221294
• RRID: AB_2879431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q5TFE4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB,IP,ChIP	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan