NT5DC1 Polyclonal antibody

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• NT5DC1 Polyclonal antibody (24102-1-AP)

NT5DC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24102-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

NT5C2L1, EC:3.1.3.-, 5'-nucleotidase domain-containing protein 1

• 

  WB analysis of MCF-7 using 24102-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 24102-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 24102-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 24102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 24102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human thyroid cancer using 24102-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human thyroid cancer using 24102-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 24102-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NT5DC1 antibody (24102-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF7 cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human lung cancer tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24102-1-AP targets NT5DC1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20916

  Product name: Recombinant human NT5DC1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-297 aa of BC015138

  Sequence: WKDIVAAIQHNYKMSAFKENCGIYFPEIKRDPGRYLHSCPESVKKWLRQLKNAGKILLLITSSHSDYCRLLCEYILGNDFTDLFDIVITNALKPGFFSHLPSQRPFRTLENDEEQEALPSLDKPGWYSQGNAVHLYELLKKMTGKPEPKVVYFGDSMHSDIFPARHYSNWETVLILEELRGDEGTRSQRPEESEPLEKKGKYEGPKAKPLNTSSKKWGSFFIDSVLGLENTEDSLVYTWSCKRISTYSTIAIPSIEAIAELPLDYKFTRFSSSNSKTAGYYPNPPLVLSSDETLISK

• Full Name: 5'-nucleotidase domain containing 1
• Calculated Molecular Weight: 455 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC015138
• Gene Symbol: NT5DC1
• Gene ID (NCBI): 221294
• RRID: AB_2879431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q5TFE4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The NT5DC1 Polyclonal antibody (Cat# 24102-1-AP) has 1 total citation. It appears in Nature Communications (Impact Factor 18.1), in a study investigating how PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression. The antibody was used for WB, IP, and ChIP applications. The research field is developmental biology and stem cell gene regulation.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB,IP,ChIP	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan