CD73 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD73 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

49

Reviews (2)

Cat no : 12231-1-AP

Synonyms

NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73)

---

Validation Data Gallery

• 

  WB analysis using 12231-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12231-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 12231-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12231-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12231-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 12231-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 12231-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human appendicitis using 12231-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: A375 cells, human spleen tissue, K-562 cells, mouse brain tissue, HT-29 cells, HuH-7 cells, Jurkat cells
• Positive IHC detected in: human lung cancer tissue, human stomach cancer tissue, human stomach tissue, mouse kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse small intestine tissue, human appendicitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 26 publications below
• IHC: See 14 publications below
• IF: See 17 publications below
• ELISA: See 1 publications below
• FC: See 5 publications below

Product Information

12231-1-AP targets CD73 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD73 fusion protein Ag2869
• Full Name: 5'-nucleotidase, ecto (CD73)
• Calculated Molecular Weight: 29 kDa, 63 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC015940
• Gene Symbol: CD73
• Gene ID (NCBI): 4907
• RRID: AB_2154081
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NT5E(5'-nucleotidase) is also named as NT5, NTE,CD73,ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer(PMID:17619139). It can be detected the band of 55 kDa,64 kDa, 70 kDa, 110 kDa by western blot(PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries (CALJA).

Publications

Species	Application	Title
rat	WB	Adv Healthc Mater; Dual-Barb Microneedle with JAK/STAT Inhibitor-Loaded Nanovesicles Encapsulation for Tendinopathy; Authors - Minhao Chen
human,mouse	IHC,WB,IF	Int J Biol Sci; CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence; Authors - Zhenni Liu
human	WB,IHC	Br J Cancer; Norepinephrine inhibits CD8+ T-cell infiltration and function, inducing anti-PD-1 mAb resistance in lung adenocarcinoma; Authors - Qishun Geng
mouse	IF	Theranostics; GATA4-driven miR-206-3p signatures control orofacial bone development by regulating osteogenic and osteoclastic activity.; Authors - Shuyu Guo
human	IF	Cell Mol Biol Lett; Reciprocal negative feedback between Prrx1 and miR-140-3p regulates rapid chondrogenesis in the regenerating antler; Authors - Pengfei Hu
	IHC	Theranostics; A sericin/ graphene oxide composite scaffold as a biomimetic extracellular matrix for structural and functional repair of calvarial bone.; Authors - Chao Qi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Alessandro (Verified Customer) (12-09-2023)

good product for ICC

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: ROs

FH

Neta (Verified Customer) (07-28-2022)

staining did not work

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse small intestine, fixed frozen