NT5E Recombinant monoclonal antibody, PBS Only (Capture)

• 

  Sandwich ELISA sample test of MP02481-1

  The mean CD73 concentration was determined to be 67.84 ng/mL in U-87 MG cell extract based on a 1.5 mg/mL extract load and 125.52 ng/mL in PC-3 cell extract based on a 1.1 mg/mL extract load.

• 

  Sandwich ELISA sample test of MP02481-1

  Plasma of six individual healthy human donors was measured. The human CD73 concentration of detected samples was determined to be 15.14 ng/mL with a range of 8.55 - 28.73 ng/mL.

• 

  Sandwich ELISA standard curve of MP02481-1

  Sandwich ELISA standard curve of MP02481-1, Human CD73 Recombinant Matched Antibody Pair - PBS only. 86512-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2974. 86512-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

• 

  Cytometric bead array sample test of MP02481-1

  The mean CD73 concentration was determined to be 895.3 ng/mL in U-87 MG cell extract based on a 1.5 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP02481-1

  Cytometric bead array standard curve of MP02481-1, CD73 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86512-2-PBS. Detection antibody: 86512-1-PBS. Standard: Eg2974. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 86512-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 86512-2-PBS against Human NT5E was performed. The affinity constant is 1.45 nM.

NT5E Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 86512-2-PBS

Clone No.251250C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

CD73, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase

---

Product Information

86512-2-PBS targets NT5E as part of a matched antibody pair:

MP02481-1: 86512-2-PBS capture and 86512-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2974

  Product name: Recombinant Human CD73 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 27-547 aa of NM_002526.3

  Sequence: WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

• Full Name: 5'-nucleotidase, ecto (CD73)
• Calculated Molecular Weight: 63 kDa
• GenBank Accession Number: NM_002526.3
• Gene Symbol: CD73
• Gene ID (NCBI): 4907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21589-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.