NT5M Polyclonal antibody

NT5M Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20765-1-AP

Synonyms

dNT-2, DNT2, dNT 2, Deoxy-5'-nucleotidase 2, 5',3'-nucleotidase, mitochondrial

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Validation Data Gallery

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  WB analysis of mouse brain using 20765-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20765-1-AP (NT5M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20765-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20765-1-AP (NT5M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 20765-1-AP

  IP result of anti-NT5M (IP:20765-1-AP, 4ug; Detection:20765-1-AP 1:600) with mouse skeletal muscle tissue lysate 2200ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20765-1-AP targets NT5M in WB, IP, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NT5M fusion protein Ag14743
• Full Name: 5',3'-nucleotidase, mitochondrial
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC035838
• Gene Symbol: NT5M
• Gene ID (NCBI): 56953
• RRID: AB_10733338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.