Tested Applications
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18084-1-AP targets Neurotrophin 3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12798 Product name: Recombinant human NTF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-257 aa of BC069773 Sequence: MSILFYVIFLAYLRGIQGNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPERGGPAKSAFQPVIAMDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGSPVVANRTSRRKRYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT Predict reactive species |
| Full Name | neurotrophin 3 |
| Calculated Molecular Weight | 257 aa, 29 kDa |
| GenBank Accession Number | BC069773 |
| Gene Symbol | Neurotrophin 3 |
| Gene ID (NCBI) | 4908 |
| RRID | AB_2298715 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20783 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neurotrophin-3 (NT-3) is a member of the neurotrophin family, which are growth factor proteins that play essential roles in the development and maintenance of the nervous system. NT-3 is synthesized by fibroblasts and has been extensively studied for its role in the development of neurons and neural crest-derived cells, such as melanocytes. It can bind to each of the Trk receptors (Trk-A, Trk-B, and Trk-C), but it primarily functions by binding to Trk-C, modulating intracellular signal transduction through the MAPK and PI3K-Akt pathways, which regulate melanocyte differentiation and survival.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Neurotrophin 3 antibody 18084-1-AP | Download protocol |
| IHC protocol for Neurotrophin 3 antibody 18084-1-AP | Download protocol |
| WB protocol for Neurotrophin 3 antibody 18084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Neurotrophin 3 Polyclonal antibody (Cat# 18084-1-AP) has 21 citations. These appear in journals including Bioactive Materials, Phytomedicine, Journal of Orthopaedic Translation, Stem Cell Research & Therapy, ACS Applied Materials & Interfaces, Translational Psychiatry, Biomaterials Science, Investigative Ophthalmology & Visual Science, Frontiers in Pharmacology, American Journal of Sports Medicine, and Scientific Reports. Research fields span neural regeneration, spinal cord and brain injury repair, stroke, neuroprotection, peripheral nerve regeneration, cancer biomarkers, and tissue engineering.
| Species | Application | Title |
|---|---|---|
Bioact Mater Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair | ||
Phytomedicine Naringin reduces mitochondrial fragmentation and excessive mitophagy in cerebral ischemia-reperfusion injury via the S-nitrosylated Dynamin-related protein 1 (SNO-DRP1) / Leucine-rich repeat kinase 2 (LRRK2) / Mitochondrial Calcium Uniporter (MCU) pathway. | ||
J Orthop Translat Schwann-endothelial crosstalk in neurovascularized cell sheets induced host CGRP release to drive mandibular bone regeneration. | ||
ACS Appl Mater Interfaces Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy. | ||
Stem Cell Res Ther NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model. | ||
Transl Psychiatry The causal involvement of the BDNF-TrkB pathway in dentate gyrus in early-life stress-induced cognitive deficits in male mice |
