NTHL1 Polyclonal antibody

NTHL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 11154-1-AP

Synonyms

NTH1, NTHL1, OCTS3



Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, HeLa cells, K-562 cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

11154-1-AP targets NTHL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NTHL1 fusion protein Ag1631
Full Name nth endonuclease III-like 1 (E. coli)
Calculated molecular weight 34 aa, 3 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC003014
Gene symbol NTHL1
Gene ID (NCBI) 4913
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

Protocols

Product Specific Protocols
WB protocol for NTHL1 antibody 11154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

FASEB J

Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.

Authors - Catherine E Aiken
humanWB

Respir Res

Protection of pulmonary epithelial cells from oxidative stress by hMYH adenine glycosylase.

Authors - Kremer Ted M TM
humanWB,IP

NAR Cancer

The DNA repair function of BCL11A suppresses senescence and promotes continued proliferation of triple-negative breast cancer cells

Authors - Elise Vickridge