NTHL1 Polyclonal antibody

NTHL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 11154-1-AP

Synonyms

NTH1, NTHL1, OCTS3

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Validation Data Gallery

• 

  WB analysis of human brain using 11154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11154-1-AP (NTHL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11154-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11154-1-AP (NTHL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11154-1-AP (NTHL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, HeLa cells, K-562 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IP: See 1 publications below

Product Information

11154-1-AP targets NTHL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NTHL1 fusion protein Ag1631
• Full Name: nth endonuclease III-like 1 (E. coli)
• Calculated Molecular Weight: 34 aa, 3 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC003014
• Gene Symbol: NTHL1
• Gene ID (NCBI): 4913
• RRID: AB_2283005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

Publications

Species	Application	Title
rat	WB	FASEB J; Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.; Authors - Catherine E Aiken
human	WB	Respir Res; Protection of pulmonary epithelial cells from oxidative stress by hMYH adenine glycosylase.; Authors - Kremer Ted M TM
human	WB,IP	NAR Cancer; The DNA repair function of BCL11A suppresses senescence and promotes continued proliferation of triple-negative breast cancer cells; Authors - Elise Vickridge
human	WB	Nucleic Acids Res; The function of BCL11B in base excision repair contributes to its dual role as an oncogene and a haplo-insufficient tumor suppressor gene; Authors - Elise Vickridge

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (02-17-2023)

Recognises NTHL1 at 34kDa, but has non-specific bands which decrease with siRNA knock-down of NTHL1 at higher molecular weights (150kDa).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS

NTHL1 Antibody Western Blot validation (1:1000 dilution) in U2OS (Cat no:11154-1-AP)