NTHL1 Polyclonal antibody

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  WB analysis using 14918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14918-1-AP (NTHL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 14918-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14918-1-AP (NTHL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 14918-1-AP

  IP result of anti-NTHL1 (IP:14918-1-AP, 4ug; Detection:14918-1-AP 1:500) with Y79 cells lysate 2000ug.

NTHL1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 14918-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

NTH1, NTHL1, OCTS3

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Tested Applications

• Positive WB detected in: HEK-293 cells, Y79 cells, HeLa cells, K-562 cells
• Positive IP detected in: Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14918-1-AP targets NTHL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6734

  Product name: Recombinant human NTHL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2-305 aa of BC000391

  Sequence: MTALSARMLTRSRSLGPGAGPRGCREEPGPLRRREAAAEARKSHSPVKRPRKAQRLRVAYEGSDSEKGEGAEPLKVPVWEPQDWQQQLVNIRAMRNKKDAPVDHLGTEHCYDSSAPPKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVDSILQTDDATLGKLIYPVGFWRSKVKYIKQTSAILQQHYGGDIPASVAELVALPGVGPKMAHLAMAVAWGTVSGIAVDTHVHRIANRLRWTKKATKSPEETRAALEEWLPRELWHEINGLLVGFGQQTCLPVHPRCHACLNQALCPAAQGL

• Full Name: nth endonuclease III-like 1 (E. coli)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000391
• Gene Symbol: NTHL1
• Gene ID (NCBI): 4913
• RRID: AB_2154555
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78549
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.; Authors - Shangming Tang
human	WB	Nat Commun; Mitochondrial respiratory function is preserved under cysteine starvation via glutathione catabolism in NSCLC; Authors - Nathan P Ward