Featured Product
KD/KO Validated

NTM Polyclonal antibody

NTM Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14373-1-AP

Publications (1)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

HNT, IgLON family member 2, IGLON2, neurotrimin, NT, NTM, NTRI

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHC

WB analysis of SH-SY5Y using 14373-1-AP
WB result of NTM antibody (14373-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NTM transfected SH-SY5Y cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of SH-SY5Y using 14373-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14373-1-AP (NTM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 14373-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14373-1-AP (NTM Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 14373-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14373-1-AP (NTM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 14373-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14373-1-AP (NTM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for NTM in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, mouse brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14373-1-AP targets NTM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5746 Product name: Recombinant human NTM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-316 aa of BC050716 Sequence: VCGYLFLPWKCLVVVSLRLLFLVPTGVPVRSGDATFPKAMDNVTVRQGESATLRCTIDNRVTRVAWLNRSTILYAGNDKWCLDPRVVLLSNTQTQYSIEIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVSPKIVEISSDISINEGNNISLTCIATGRPEPTVTWRHISPKAVGFVSEDEYLEIQGITREQSGDYECSASNDVAAPVVRRVKVTVNYPPYISEAKGTGVPVGQKGTLQCEASAVPSAEFQWYKDDKRLIEGKKGVKVENRPFLSKLIFFNVSEHDYGNYTCVASNKLGHTNASIMLFGETVL Predict reactive species
Full Name	neurotrimin
Calculated Molecular Weight	38 kDa
Observed Molecular Weight	58 kDa, 65 kDa
GenBank Accession Number	BC050716
Gene Symbol	NTM
Gene ID (NCBI)	50863
RRID	AB_2878048
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9P121
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neurotrimin (NTM) is a glycosylphosphatidylinositol (GPI)-anchored neural cell adhesion molecule. Neurotrimin together with the limbic system-associated protein (LAMP) and the opioid binding cell adhesion molecule (OBCAM) comprise the IgLON family of the immunoglobulin superfamily. Neurotrimin may be promoting neurite outgrowth and adhesion via a homophilic mechanism.

Protocols

Product Specific Protocols
IHC protocol for NTM antibody 14373-1-AP	Download protocol
WB protocol for NTM antibody 14373-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The anti-NTM (Neurotrimin) polyclonal antibody, catalog number 14373-1-AP, has a total of 1 citation. It was cited in the Journal of Investigative Dermatology (IF: 7) in a 2025 Mendelian randomization study identifying potential drug targets for keloid. The antibody was used in WB and IF applications on human samples. The associated research field is dermatology, specifically keloid pathogenesis and drug target discovery.