NTN1-Specific Polyclonal antibody

NTN1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 20235-1-AP

Synonyms

netrin 1, NTN1, NTN1L, NTN1-Specific

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Validation Data Gallery

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  WB analysis using 20235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20235-1-AP (NTN1-Specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20235-1-AP (NTN1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 20235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20235-1-AP (NTN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20235-1-AP (NTN1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse brain tissue, rat heart tissue, rat kidney tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

20235-1-AP targets NTN1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: netrin 1
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: NM_004822
• Gene Symbol: NTN1
• Gene ID (NCBI): 9423
• RRID: AB_2918065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NTN1 is also named as NTN1L. Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. NTN1 is involved in colorectal tumorigenesis by regulating apoptosis. 20235-1-AP antibody detects 65-68 kDa (3 kDa signal peptide) protein in SDS-PAGE. This antibody is specific to NTN1.

Publications

Species	Application	Title
human	WB	J Diabetes Res; High-Glucose-Induced Injury to Proximal Tubules of the Renal System Is Alleviated by Netrin-1 Suppression of Akt/mTOR; Authors - Chenxiao Liu
human	WB	Int J Oncol; Netrin‑1 interference potentiates epithelial‑to‑mesenchymal transition through the PI3K/AKT pathway under the hypoxic microenvironment conditions of non‑small cell lung cancer.; Authors - Xiayun Jin
human	WB,IHC	J Int Med Res; Detection of netrin-1 as a novel biomarker for diagnosis and chemotherapeutic monitoring of lung cancer.; Authors - Yuanyuan Zhao
human,mouse	WB	Tissue Cell; Exosomes derived from human umbilical cord mesenchymal stem cells loaded with RVG-Lamp2b and Netrin-1 promotes Schwann cell invasion and migration; Authors - Jie He