NTN1-Specific Recombinant monoclonal antibody

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  WB analysis using 85375-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85375-1-RR (NTN1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 85375-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NTN1-Specific antibody (85375-1-RR, Clone: 242409B11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NTN1-Specific Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85375-1-RR

Clone No.242409B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NTN1, Epididymis tissue protein Li 131P, netrin 1, Netrin-1, NTN1L

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Tested Applications

• Positive WB detected in: IMR-32 cells, SH-SY5Y cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85375-1-RR targets NTN1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: netrin 1
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: NM_004822
• Gene Symbol: NTN1
• Gene ID (NCBI): 9423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95631
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NTN1 is also named as NTN1L. Netrins control the guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serves as a survival factor through its association with its receptors, which prevents the initiation of apoptosis. NTN1 is involved in colorectal tumorigenesis by regulating apoptosis.