TrkB Polyclonal antibody

TrkB Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, Peptide array, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13129-1-AP

Synonyms

GP145 TrkB, NTRK2, TrkB, TrkB tyrosine kinase



Tested Applications

Positive WB detected inmouse brain tissue, mouse spinal cord tissue, pig cerebellum tissue, human cerebellum tissue, human stomach tissue, rat brain tissue, mouse cerebellum tissue
Positive IP detected inmouse cerebellum tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13129-1-AP targets TrkB in WB, Peptide array, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TrkB fusion protein Ag3790
Full Name neurotrophic tyrosine kinase, receptor, type 2
Calculated molecular weight 822 aa, 92 kDa
Observed molecular weight 90-95 kDa, 140-145 kDa
GenBank accession numberBC031835
Gene symbol NTRK2
Gene ID (NCBI) 4915
RRIDAB_2155156
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).

Protocols

Product Specific Protocols
WB protocol for TrkB antibody 13129-1-APDownload protocol
IHC protocol for TrkB antibody 13129-1-APDownload protocol
IF protocol for TrkB antibody 13129-1-APDownload protocol
IP protocol for TrkB antibody 13129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Chemosphere

Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.

Authors - Mingdan You
ratWB

Oxid Med Cell Longev

Dl-3-n-Butylphthalide Improves Neuroinflammation in Mice with Repeated Cerebral Ischemia-Reperfusion Injury through the Nrf2-Mediated Antioxidant Response and TLR4/MyD88/NF-κB Signaling Pathway.

Authors - Yaran Gao
mouseWB

J Affect Disord

Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel

Authors - Hui Yao
ratWB

ACS Chem Neurosci

Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine.

Authors - Lian Liu
humanIHC

Am J Cancer Res

LMP1 promotes nasopharyngeal carcinoma metastasis through NTRK2-mediated anoikis resistance.

Authors - Zhilan Li
humanWB,IF

Cell Biosci

A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.

Authors - Juan Yin