Validation Data Gallery
|Positive WB detected in
|mouse brain tissue, mouse spinal cord tissue, pig cerebellum tissue, human cerebellum tissue, human stomach tissue, rat brain tissue, mouse cerebellum tissue
|Positive IP detected in
|mouse cerebellum tissue
|Positive IHC detected in
|mouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:6000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:300-1:1200
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
13129-1-AP targets TrkB in WB, Peptide array, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|human, mouse, rat, pig
|human, mouse, rat, zebrafish
|Host / Isotype
|Rabbit / IgG
|TrkB fusion protein Ag3790
|neurotrophic tyrosine kinase, receptor, type 2
|Calculated molecular weight
|822 aa, 92 kDa
|Observed molecular weight
|90-95 kDa, 140-145 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).
|Product Specific Protocols
|WB protocol for TrkB antibody 13129-1-AP
|IHC protocol for TrkB antibody 13129-1-AP
|IF protocol for TrkB antibody 13129-1-AP
|IP protocol for TrkB antibody 13129-1-AP
|Click here to view our Standard Protocols
Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.
Oxid Med Cell Longev
Dl-3-n-Butylphthalide Improves Neuroinflammation in Mice with Repeated Cerebral Ischemia-Reperfusion Injury through the Nrf2-Mediated Antioxidant Response and TLR4/MyD88/NF-κB Signaling Pathway.
J Affect Disord
Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel
ACS Chem Neurosci
Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine.
Am J Cancer Res
LMP1 promotes nasopharyngeal carcinoma metastasis through NTRK2-mediated anoikis resistance.
A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.