TrkB Polyclonal antibody
TrkB Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig and More (1)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13129-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, mouse spinal cord tissue, pig cerebellum tissue, human cerebellum tissue, human stomach tissue, rat brain tissue, mouse cerebellum tissue |
Positive IP detected in | mouse cerebellum tissue |
Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 38 publications below |
IHC | See 5 publications below |
IF | See 3 publications below |
CoIP | See 1 publications below |
Product Information
13129-1-AP targets TrkB in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TrkB fusion protein Ag3790 |
Full Name | neurotrophic tyrosine kinase, receptor, type 2 |
Calculated molecular weight | 822 aa, 92 kDa |
Observed molecular weight | 90-95 kDa, 140-145 kDa |
GenBank accession number | BC031835 |
Gene symbol | NTRK2 |
Gene ID (NCBI) | 4915 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).
Protocols
Product Specific Protocols | |
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WB protocol for TrkB antibody 13129-1-AP | Download protocol |
IHC protocol for TrkB antibody 13129-1-AP | Download protocol |
IF protocol for TrkB antibody 13129-1-AP | Download protocol |
IP protocol for TrkB antibody 13129-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Affect Disord Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel | ||
Front Aging Neurosci Effect of β-hydroxybutyrate on behavioral alterations, molecular and morphological changes in CNS of multiple sclerosis mouse model | ||
Am J Cancer Res LMP1 promotes nasopharyngeal carcinoma metastasis through NTRK2-mediated anoikis resistance. | ||
Cell Biosci A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation. | ||
Ecotoxicol Environ Saf Perinatal exposure to BDE-47 exacerbated autistic-like behaviors and impairments of dendritic development in a valproic acid-induced rat model of autism. | ||
Front Pharmacol Jiedu Tongluo Granules Ameliorates Post-stroke Depression Rat Model via Regulating NMDAR/BDNF Signaling Pathway. |