TrkB Polyclonal antibody

TrkB Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 13129-1-AP

Synonyms

GP145 TrkB, NTRK2, TrkB, TrkB tyrosine kinase



Tested Applications

Positive WB detected inpig cerebellum tissue, human cerebellum tissue, human stomach tissue, mouse spinal cord tissue, mouse cerebellum tissue
Positive IP detected inmouse cerebellum tissue
Positive IHC detected inmouse cerebellum tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13129-1-AP targets TrkB in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TrkB fusion protein Ag3790
Full Name neurotrophic tyrosine kinase, receptor, type 2
Calculated molecular weight 822 aa, 92 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC031835
Gene symbol NTRK2
Gene ID (NCBI) 4915
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TrkB antibody 13129-1-APDownload protocol
IHC protocol for TrkB antibody 13129-1-APDownload protocol
IF protocol for TrkB antibody 13129-1-APDownload protocol
IP protocol for TrkB antibody 13129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Life Sci

Hyperbaric oxygen therapy reduces apoptosis and dendritic/synaptic degeneration via the BDNF/TrkB signaling pathways in SCI rats.

Authors - Xinwang Ying
mouseWB

Biomed Pharmacother

Chaihu-Shugan-San and absorbed meranzin hydrate induce anti-atherosclerosis and behavioral improvements in high-fat diet ApoE-/- mice via anti-inflammatory and BDNF-TrkB pathway.

Authors - Lan Li
ratWB

Chemosphere

Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.

Authors - Mingdan You
zebrafishWB

Fish Shellfish Immunol

Activation of BDNF-TrkB signaling pathway-regulated brain inflammation in pentylenetetrazole-induced seizures in zebrafish.

Authors - Meng Jin
mouseWB

Biochim Biophys Acta Mol Basis Dis

PMID: 30389579

Authors - Chunling Yuan
mouseWB,CoIP

Cell Death Dis

Alpha-synuclein is involved in manganese-induced spatial memory and synaptic plasticity impairments via TrkB/Akt/Fyn-mediated phosphorylation of NMDA receptors.

Authors - Zhuo Ma