TrkB Polyclonal antibody

TrkB Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, pig and More (1)


WB, IP, IF, IHC, CoIP, ELISA, Peptide array



Cat no : 13129-1-AP


GP145 TrkB, NTRK2, TrkB, TrkB tyrosine kinase

Tested Applications

Positive WB detected inmouse brain tissue, mouse spinal cord tissue, pig cerebellum tissue, human cerebellum tissue, human stomach tissue, rat brain tissue, mouse cerebellum tissue
Positive IP detected inmouse cerebellum tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13129-1-AP targets TrkB in WB, IP, IF, IHC, CoIP, ELISA, Peptide array applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TrkB fusion protein Ag3790
Full Name neurotrophic tyrosine kinase, receptor, type 2
Calculated Molecular Weight 822 aa, 92 kDa
Observed Molecular Weight 90-95 kDa, 140-145 kDa
GenBank Accession NumberBC031835
Gene Symbol TrkB
Gene ID (NCBI) 4915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).


Product Specific Protocols
WB protocol for TrkB antibody 13129-1-APDownload protocol
IHC protocol for TrkB antibody 13129-1-APDownload protocol
IF protocol for TrkB antibody 13129-1-APDownload protocol
IP protocol for TrkB antibody 13129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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