TrkC Polyclonal antibody

TrkC Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11999-1-AP

Synonyms

GP145 TrkC, gp145(trkC), NT 3 growth factor receptor, NTRK3, TrkC, TrkC tyrosine kinase

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Validation Data Gallery

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  WB analysis of fetal human brain using 11999-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11999-1-AP (TrkC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11999-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11999-1-AP (TrkC antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11999-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11999-1-AP (TrkC antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 11999-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11999-1-AP (TrkC antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 11999-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11999-1-AP (TrkC antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 11999-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 11999-1-AP (TrkC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, rat brain tissue, mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

11999-1-AP targets TrkC in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TrkC fusion protein Ag2635
• Full Name: neurotrophic tyrosine kinase, receptor, type 3
• Calculated Molecular Weight: 839 aa, 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC013693
• Gene Symbol: NTRK3
• Gene ID (NCBI): 4916
• RRID: AB_2155428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Identification of New miRNA-mRNA Networks in the Development of Non-syndromic Cleft Lip With or Without Cleft Palate.; Authors - Chengyi Fu
human	WB	Stem Cell Res Ther; NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model.; Authors - Ke Wu
mouse	WB	iScience; Antispasmodic Drug Drofenine as an Inhibitor of Kv2.1 Channel Ameliorates Peripheral Neuropathy in Diabetic Mice.; Authors - Xiaoju Xu

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