TrkC Monoclonal antibody

TrkC Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2A7F11

Cat no : 66380-1-Ig

Synonyms

GP145 TrkC, gp145(trkC), NT 3 growth factor receptor, NTRK3, Trk C, TRKC, TrkC tyrosine kinase

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Validation Data Gallery

• 

  WB analysis using 66380-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66380-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 66380-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 66380-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of SH-SY5Y using 66380-1-Ig

  1X10^6 SH-SY5Y cells were stained with 0.20 ug Anti-Human TrkC (66380-1-Ig, Clone:2A7F11) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: pig brain tissue, SH-SY5Y cells, PC-12 cells, fetal human brain tissue, rat brain tissue, mouse brain tissue
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

66380-1-Ig targets TrkC in WB, IF, FC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

• Tested Reactivity: human, rat, mouse, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TrkC fusion protein Ag2635
• Full Name: neurotrophic tyrosine kinase, receptor, type 3
• Calculated Molecular Weight: 839 aa, 94 kDa
• Observed Molecular Weight: 110 kDa, 160 kDa
• GenBank Accession Number: BC013693
• Gene Symbol: NTRK3
• Gene ID (NCBI): 4916
• RRID: AB_2881756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Bioact Mater; Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair; Authors - Zhaoping Wu

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