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TrkC Monoclonal antibody

TrkC Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2A7F11

Cat no : 66380-1-Ig

Synonyms

GP145 TrkC, gp145(trkC), NT 3 growth factor receptor, NTRK3, Trk C, TRKC, TrkC tyrosine kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TrkC Monoclonal antibody (66380-1-Ig)

    WB analysis using 66380-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using TrkC Monoclonal antibody (66380-1-Ig)

    WB analysis of fetal human brain using 66380-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using TrkC Monoclonal antibody (66380-1-Ig)

    WB analysis of SH-SY5Y using 66380-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using TrkC Monoclonal antibody (66380-1-Ig)

    WB analysis of PC-12 using 66380-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using TrkC Monoclonal antibody (66380-1-Ig)

    FC experiment of SH-SY5Y using 66380-1-Ig

    1X10^6 SH-SY5Y cells were stained with 0.20 ug Anti-Human TrkC (66380-1-Ig, Clone:2A7F11) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

Tested Applications

Positive WB detected inpig brain tissue, fetal human brain tissue, PC-12 cells, SH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

66380-1-Ig targets TrkC in WB, FC,ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TrkC fusion protein Ag2635
Full Name neurotrophic tyrosine kinase, receptor, type 3
Calculated molecular weight 839 aa, 94 kDa
Observed molecular weight 110 kDa, 160 kDa
GenBank accession numberBC013693
Gene symbol NTRK3
Gene ID (NCBI) 4916
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TrkC antibody 66380-1-IgDownload protocol
FC protocol for TrkC antibody 66380-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols