Tested Applications
| Positive WB detected in | mouse brain tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | human colon cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22723-1-AP targets ARK5 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18612 Product name: Recombinant human NUAK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 515-662 aa of BC152462 Sequence: HSLSCRRKGILKHSSKYSAGTMDPALVSPEMPTLESLSEPGVPAEGLSRSYSRPSSVISDDSVLSSDSFDLLDLQENRPARQRIRSCVSAENFLQIQDFEGLQNRPRPQYLKRYRNRLADSSFSLLTDMDDVTQVYKQALEICSKLN Predict reactive species |
| Full Name | NUAK family, SNF1-like kinase, 1 |
| Calculated Molecular Weight | 661 aa, 74 kDa |
| Observed Molecular Weight | 74 kDa |
| GenBank Accession Number | BC152462 |
| Gene Symbol | ARK5 |
| Gene ID (NCBI) | 9891 |
| RRID | AB_2879157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | O60285 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NUAK1 is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. It is also named as ARK5, KIAA0537, OMPHK1 and belongs to the protein kinase superfamily. The physiological role of NUAK1 is potentially to suppress glucose uptake through negative regulation of INS signaling in oxidative muscle. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARK5 antibody 22723-1-AP | Download protocol |
| WB protocol for ARK5 antibody 22723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-NUAK1 antibody (Cat# 22723-1-AP) has 16 total citations spanning journals including Cell Reports, Acta Neuropathologica, Molecular Medicine, Frontiers in Immunology, Cancer Cell International, PLoS Genetics, and Scientific Reports. Research fields covered include hepatocellular carcinoma, gastric cancer, breast cancer, esophageal squamous cell carcinoma, rectal cancer, pancreatic cancer, cervical cancer, Alzheimer's disease, neurobiology, and Plasmodium falciparum infection. Applications cited include WB, IHC, IF, and CoIP.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci miR-424-5p represses the metastasis and invasion of intrahepatic cholangiocarcinoma by targeting ARK5. | ||
Acta Neuropathol p-tau Ser356 is associated with Alzheimer's disease pathology and is lowered in brain slice cultures using the NUAK inhibitor WZ4003 | ||
Mol Med NUAK1 acts as a novel regulator of PD-L1 via activating GSK-3β/β-catenin pathway in hepatocellular carcinoma | ||
Cell Rep NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer
| ||
Cancer Cell Int NUAK1 promotes tumor metastasis through upregulating slug transcription in esophageal squamous cell carcinoma
| ||
Front Immunol Identification of senescence-related subtypes, establishment of a prognosis model, and characterization of a tumor microenvironment infiltration in breast cancer
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