ARK5 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARK5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 22723-1-AP

Synonyms

AMPK related protein kinase 5, ARK5, KIAA0537, NUAK family SNF1 like kinase 1, NUAK1

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Validation Data Gallery

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  WB analysis using 22723-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 22723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 22723-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22723-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat heart tissue, mouse heart tissue
• Positive IHC detected in: human colon cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Product Information

22723-1-AP targets ARK5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARK5 fusion protein Ag18612
• Full Name: NUAK family, SNF1-like kinase, 1
• Calculated Molecular Weight: 661 aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC152462
• Gene Symbol: ARK5
• Gene ID (NCBI): 9891
• RRID: AB_2879157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUAK1 is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. It is also named as ARK5, KIAA0537, OMPHK1 and belongs to the protein kinase superfamily. The physiological role of NUAK1 is potentially to suppress glucose uptake through negative regulation of INS signaling in oxidative muscle. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	PLoS Genet; A Mouse Model Uncovers LKB1 as an UVB-Induced DNA Damage Sensor Mediating CDKN1A (p21WAF1/CIP1) Degradation.; Authors - Rosaura Esteve-Puig ; KD Validated
human	IHC	Front Immunol; Identification of senescence-related subtypes, establishment of a prognosis model, and characterization of a tumor microenvironment infiltration in breast cancer; Authors - Yanling Zhou ; KD Validated
human	WB	Biomed Pharmacother; Discovery of a novel NUAK1 inhibitor against pancreatic cancer.; Authors - Myeong-Seong Seo
human	IHC	Int J Biol Sci; miR-424-5p represses the metastasis and invasion of intrahepatic cholangiocarcinoma by targeting ARK5.; Authors - Jingbang Wu
mouse	IF	Sci Rep; High-throughput kinase inhibitor screening reveals roles for Aurora and Nuak kinases in neurite initiation and dendritic branching.; Authors - Sara M Blazejewski
human	WB	Int J Immunopathol Pharmacol; Cancer susceptibility 18 positively regulates NUAK Family Kinase 1 expression to promote migration and invasion via sponging of miR-5586-5p in cervical cancer cells; Authors - Jingrong Wang