MTH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16705-1-AP

Synonyms

8 oxo dGTPase, MTH 1, MTH1, Nudix motif 1, NUDT1

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Validation Data Gallery

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  WB analysis of HepG2 using 16705-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16705-1-AP (MTH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16705-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16705-1-AP (MTH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16705-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16705-1-AP (MTH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 16705-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16705-1-AP (MTH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16705-1-AP (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 16705-1-AP

  Immunofluorescent analysis of PC-3 cells using 16705-1-AP (MTH1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, PC-3 cells
• Positive IHC detected in: human lung cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

16705-1-AP targets MTH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTH1 fusion protein Ag10103
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 1
• Calculated Molecular Weight: 179aa,20 kDa; 197aa,23 kDa
• Observed Molecular Weight: 18-24 kDa
• GenBank Accession Number: BC014618
• Gene Symbol: MTH1
• Gene ID (NCBI): 4521
• RRID: AB_2153822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.

Publications

Species	Application	Title
human	WB	Oncotarget; Farnesyl phenolic enantiomers as natural MTH1 inhibitors from Ganoderma sinense.; Authors - Ya Gao ; KD Validated
mouse	WB	Colloids Surf B Biointerfaces; Ultrasound-triggered reactive oxygen species effector nanoamplifier for enhanced combination therapy of mutant p53 tumors.; Authors - Xiang Wang
mouse	WB	Photodiagnosis Photodyn Ther; Cepharanthine synergizes with photodynamic therapy for boosting ROS-driven DNA damage and suppressing MTH1 as a potential anti-cancer strategy; Authors - Zi-Yi Yang
human	WB	BMC Gastroenterol; USP9X regulates the proliferation, survival, migration and invasion of gastric cancer cells by stabilizing MTH1; Authors - Wenji Xu