NUDT3/4/10/11 Polyclonal antibody

NUDT3/4/10/11 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17508-1-AP

Synonyms

NUDT10, DIPP3A, Diphosphoinositol polyphosphate phosphohydrolase 3-alpha, Diadenosine hexaphosphate hydrolase, Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-alpha

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Validation Data Gallery

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  WB analysis using 17508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17508-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17508-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17508-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17508-1-AP

  Immunofluorescent analysis of Hela cells, using NUDT10 antibody 17508-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17508-1-AP targets NUDT3/4/10/11 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDT3/4/10/11 fusion protein Ag11711
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 10
• Calculated Molecular Weight: 164 aa, 19 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC050700
• Gene Symbol: NUDT10
• Gene ID (NCBI): 170685
• RRID: AB_2154145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT10 (nucleoside diphosphate linked moiety X-type motif 10), also named as DIPP3A or APS2, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).