NUDT12 Polyclonal antibody

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  WB analysis using 17487-1-AP

  mouse liver tissues were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17487-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17487-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NUDT12 antibody (17487-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 17487-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NUDT12 antibody (17487-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NUDT12 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17487-1-AP

Publications(1)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

DeNADding enzyme NUDT12, EC:3.6.1.-, EC:3.6.1.22, NAD-capped RNA hydrolase NUDT12, NADH pyrophosphatase NUDT12

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells
• Positive IF/ICC detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17487-1-AP targets NUDT12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDT12 fusion protein Ag11557
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 12
• Calculated Molecular Weight: 462 aa, 52 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC041099
• Gene Symbol: NUDT12
• Gene ID (NCBI): 83594
• RRID: AB_2236164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BQG2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT12 is a member of the Nudix superfamily of pyrophosphohydrolases that has a broad range of substrates, including (d)NTPs, nucleotides, capped RNAs, dinucleotide coenzymes (NAD, FAD, dpCoA), and their derivatives. NUDT12 is active only as homodimers, with each monomer contributing to creation of the two functional catalytic pockets (PMID: 37301333, 31875550). The NUDT12 gene on chromosome 5q21 encodes the open reading frame of the putative 462-amino-acid, 52 kDa NUDT12 protein (PMID: 12790796).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Dis; A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells.; Authors - Hiroaki Konishi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-19-2025)

Great antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

NUDT12 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:17487-1-AP)

FH

Tsimafei (Verified Customer) (11-19-2024)

Antibody detect a band with the expected molecular weight of Nudt12

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS

NUDT12 Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:17487-1-AP)

FH

Moritz (Verified Customer) (09-12-2024)

worked well for detecting NUDT12 in WB. band was specific and slightly below 50kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

NUDT12 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:17487-1-AP)