NUDT15 Polyclonal antibody

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  WB analysis using 25914-1-AP

  Jurkat lysates were subjected to SDS PAGE followed by western blot with 25914-1-AP (NUDT15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25914-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25914-1-AP (NUDT15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

NUDT15 Polyclonal Antibody for WB, ELISA

Cat No. 25914-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

8 oxo dGTPase NUDT15, EC:3.6.1.9, MTH2, MutT homolog 2, Nucleoside diphosphate-linked moiety X motif 15

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25914-1-AP targets NUDT15 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23242

  Product name: Recombinant human NUDT15 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-164 aa of BC133015

  Sequence: MTASAQPRGRRPGVGVGVVVTSCKHPRCVLLGKRKGSVGAGSFQLPGGHLEFGETWEECAQRETWEEAALHLKNVHFASVVNSFIEKENYHYVTILMKGEVDVTHDSEPKNVEPEKNESWEWVPWEELPPLDQLFWGLRCLKEQGYDPFKEDLNHLVGYKGNHL

• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 15
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC133015
• Gene Symbol: NUDT15
• Gene ID (NCBI): 55270
• RRID: AB_2880294
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NV35
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT15 (nucleoside diphosphate-linked moiety X-type motif 15) is homologues to NUDT1 (MutT homologue 1, MTH1) and thus has previously been referred to as MTH2. NUDT15 is a key enzyme in thiopurine metabolism and tempers the activity of thiopurine drugs by catalyzing the hydrolysis of 6-thio-dGTP and 6-thio-GTP (PMID: 27530327, 32690945). NUDT15 is a 164-amino-acid protein that belongs to the nudix hydrolase enzyme family, whose members are capable of hydrolyzing compounds with the general structure of a nucleoside diphosphate linked to another moiety, X. (PMID: 25108385)