NUDT19 Polyclonal antibody

NUDT19 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55126-1-AP

Synonyms

Nudix motif 19, NUDT19, RP2

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 55126-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55126-1-AP (NUDT19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55126-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55126-1-AP (NUDT19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55126-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55126-1-AP (NUDT19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 55126-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55126-1-AP (NUDT19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 55126-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55126-1-AP (NUDT19 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 55126-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55126-1-AP (NUDT19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 55126-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55126-1-AP (NUDT19 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 55126-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55126-1-AP (NUDT19 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 55126-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55126-1-AP (NUDT19 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55126-1-AP targets NUDT19 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 19
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: NM_001105570
• Gene Symbol: NUDT19
• Gene ID (NCBI): 390916
• RRID: AB_10858394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT19, also named as RP2, belongs to the Nudix hydrolase family. It is a coenzyme A diphosphatase that mediates the hydrolysis of a wide range of CoA esters, including choloyl-CoA and branched-chain fatty-acyl-CoA esters. The antibody is specific to NUDT19.