NUDT2 Polyclonal antibody

NUDT2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10484-1-AP

Synonyms

Ap4A hydrolase, Ap4Aase, APAH1, Diadenosine tetraphosphatase, Nudix motif 2, NUDT2

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Validation Data Gallery

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  WB analysis using 10484-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10484-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:4000 incubated at 4 degree celsius over night.

• 

  WB analysis using 10484-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10484-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 10484-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10484-1-AP

  IP result of anti-NUDT2 (IP:10484-1-AP, 4ug; Detection:10484-1-AP 1:1000) with HEK-293 cells lysate 2600 ug.

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  IHC staining of human breast cancer using 10484-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10484-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10484-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10484-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse spleen tissue, mouse colon tissue, mouse liver tissue, rat liver tissue, mouse brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

10484-1-AP targets NUDT2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDT2 fusion protein Ag0743
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 2
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC004926
• Gene Symbol: NUDT2
• Gene ID (NCBI): 318
• RRID: AB_2153978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma; Authors - Sana' Hidmi ; KD Validated