Tested Applications
| Positive WB detected in | A431 cells, NIH/3T3 cells, HL-60 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10322-1-AP targets NUDT21 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0393 Product name: Recombinant human NUDT21 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-222 aa of BC001403 Sequence: MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRF Predict reactive species |
| Full Name | nudix (nucleoside diphosphate linked moiety X)-type motif 21 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC001403 |
| Gene Symbol | NUDT21 |
| Gene ID (NCBI) | 11051 |
| RRID | AB_2251496 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43809 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| IHC protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| IP protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| WB protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-CFIm25 (NUDT21) antibody, catalog number 10322-1-AP, has 55 citations spanning journals including Nature, Cell Discovery, Molecular Cell, Nucleic Acids Research, Journal of Clinical Investigation, Advanced Science, Science Advances, Cell Death & Differentiation, EMBO Journal, Cell Reports, PNAS, Oncogene, and others. Research fields cover alternative polyadenylation, cancer (glioblastoma, lung, gastric, ovarian, pancreatic, melanoma), fibrosis (pulmonary, dermal, renal, hepatic), neurogenesis, macrophage immunity, HIV infection, and RNA 3′-end processing.
| Species | Application | Title |
|---|---|---|
Nature CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.
| ||
Cell Mol Immunol Alternative mRNA polyadenylation regulates macrophage hyperactivation via the autophagy pathway | ||
Cell Discov RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation | ||
Cell Discov NUDT21 lactylation reprograms alternative polyadenylation to promote cuproptosis resistance | ||
Mol Cell Mediator regulates transcriptional termination through crosstalk with pre-mRNA 3' end processing factors | ||
Mol Cell Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors. |
Reviews
What customers say
Based on two reviews, this antibody performs well in Western blot, with one user reporting excellent results at 1:1000 dilution on nuclear protein extracts from Jurkat cells (5/5 rating). For immunofluorescence, another user tested it at 1:100 on mouse brain tissue alongside NeuN and DAPI but encountered high background issues requiring troubleshooting (3/5 rating). Overall, the antibody shows strong specificity in WB applications, while IF use may require optimization to reduce background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shahrazad (Verified Customer) (07-23-2025) | Used for IF of mouse brain samples along with NeuN and DAPI. Troubleshooting for high background problem.
|
Fabienne (Verified Customer) (05-09-2025) | it works very well in western blot (tested on nuclear protein extracts)
 |
