- Featured Product
- KD/KO Validated
NUDT21 Polyclonal antibody
NUDT21 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ChIP,ELISA
Conjugate
Unconjugated
Cat no : 10322-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, human brain tissue, HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human brain tissue, human placenta tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 17 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
10322-1-AP targets NUDT21 in WB, IP, IHC, IF, CoIP, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NUDT21 fusion protein Ag0393 |
| Full Name | nudix (nucleoside diphosphate linked moiety X)-type motif 21 |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC001403 |
| Gene symbol | NUDT21 |
| Gene ID (NCBI) | 11051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| IHC protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| IF protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| IP protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell The mRNA Export Receptor NXF1 Coordinates Transcriptional Dynamics, Alternative Polyadenylation, and mRNA Export.
| ||
J Clin Invest Cleavage factor 25 deregulation contributes to pulmonary fibrosis through alternative polyadenylation. | ||
Cancer Manag Res NUDT21 Suppresses Breast Cancer Tumorigenesis Through Regulating CPSF6 Expression.
| ||
RNA CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function.
| ||
Biochem Biophys Res Commun Suppression of cleavage factor Im 25 promotes the proliferation of lung cancer cells through alternative polyadenylation. |
