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  • KD/KO Validated

NUDT21 Polyclonal antibody

NUDT21 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 10322-1-AP

Synonyms

CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, Nudix motif 21, NUDT21



Tested Applications

Positive WB detected inA431 cells, human brain tissue, HEK-293 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10322-1-AP targets NUDT21 in WB, IP, IHC, IF, CoIP, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUDT21 fusion protein Ag0393
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 21
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC001403
Gene symbol NUDT21
Gene ID (NCBI) 11051
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.

Protocols

Product Specific Protocols
WB protocol for NUDT21 antibody 10322-1-APDownload protocol
IHC protocol for NUDT21 antibody 10322-1-APDownload protocol
IF protocol for NUDT21 antibody 10322-1-APDownload protocol
IP protocol for NUDT21 antibody 10322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

The mRNA Export Receptor NXF1 Coordinates Transcriptional Dynamics, Alternative Polyadenylation, and mRNA Export.

Authors - Suli Chen
  • KD Validated
mouseWB,IF

J Clin Invest

Cleavage factor 25 deregulation contributes to pulmonary fibrosis through alternative polyadenylation.

Authors - Tingting Weng
humanWB,IHC,IP

Cancer Manag Res

NUDT21 Suppresses Breast Cancer Tumorigenesis Through Regulating CPSF6 Expression.

Authors - Bi-Jun Wang
  • KD Validated
humanWB

RNA

CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function.

Authors - Chioniso P Masamha
  • KD Validated
human

BMC Res Notes

Effect of CFIm25 knockout on RNA polymerase II transcription.

Authors - Michael Tellier
  • KO Validated
humanWB,IF

Biochem Biophys Res Commun

Suppression of cleavage factor Im 25 promotes the proliferation of lung cancer cells through alternative polyadenylation.

Authors - Jingjing Huang