NUDT3/4/10/11 Polyclonal antibody

NUDT3/4/10/11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20542-1-AP

Synonyms

DIPP, DIPP 1, DIPP1, Nudix motif 3, NUDT3

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Validation Data Gallery

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  WB analysis using 20542-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20542-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20542-1-AP is NUDT3/4/10/11 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDT3/4/10/11 fusion protein Ag14587
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 3
• Calculated Molecular Weight: 172 aa, 19 kDa
• Observed Molecular Weight: 20-30 kDa
• GenBank Accession Number: BC007727
• Gene Symbol: NUDT3
• Gene ID (NCBI): 11165
• RRID: AB_10696178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT3 (nucleoside diphosphate linked moiety X-type motif 3), also named as DIPP1, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).