NUDT9 Polyclonal antibody

NUDT9 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15068-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ADP ribose diphosphatase, ADP ribose phosphohydrolase, ADPR PPase, Nudix motif 9, NUDT10, NUDT9, UNQ3012/PRO9771

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 15068-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15068-1-AP (NUDT9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Raji using 15068-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 15068-1-AP (NUDT9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 15068-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15068-1-AP (NUDT9 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 15068-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15068-1-AP (NUDT9 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 15068-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15068-1-AP (NUDT9 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 15068-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15068-1-AP (NUDT9 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, Raji cells, rat brain tissue
Positive IHC detected in	human heart tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15068-1-AP targets NUDT9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7075 Product name: Recombinant human NUDT9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-350 aa of BC000542 Sequence: RCRGIQAFRNSFSSSWFHLNTNVMSGSNGSKENSHNKARTSPYPGSKVERSQVPNEKVGWLVEWQDYKPVEYTAVSVLAGPRWADPQISESNFSPKFNEKDGHVERKSKNGLYEIENGRPRNPAGRTGLVGRGLLGRWGPNHAADPIITRWKRDSSGNKIMHPVSGKHILQFVAIKRKDCGEWAIPGGMVDPGEKISATLKREFGEEALNSLQKTSAEKREIEEKLHKLFSQDHLVIYKGYVDDPRNTDNAWMETEAVNYHDETGEIMDNLMLEAGDDAGKVKWVDINDKLKLYASHSQFIKLVAEKRDAHWSEDSEADCHAL Predict reactive species
Full Name	nudix (nucleoside diphosphate linked moiety X)-type motif 9
Calculated Molecular Weight	39 kDa
Observed Molecular Weight	39 kDa
GenBank Accession Number	BC000542
Gene Symbol	NUDT9
Gene ID (NCBI)	53343
RRID	AB_10951855
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BW91
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for NUDT9 antibody 15068-1-AP	Download protocol
WB protocol for NUDT9 antibody 15068-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The NUDT9 Polyclonal antibody (Cat# 15068-1-AP) has a total of 2 citations, both published in Scientific Reports. The cited studies span two research fields: one investigates metabolic reprogramming in hepatocellular carcinoma using quantitative proteomics (SWATH-MS), while the other examines the roles of TRPM2 and TRPV1 channels in hyperalgesia, apoptosis, and oxidative stress in a rat fibromyalgia model. Both studies utilized the antibody for Western blot applications in human and rat samples, respectively.

Species	Application	Title
rat	WB	Sci Rep Involvement of TRPM2 and TRPV1 channels on hyperalgesia, apoptosis and oxidative stress in rat fibromyalgia model: Protective role of selenium. Authors - Esra Yüksel View Article
human	WB	Sci Rep Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues. Authors - Yanyan Gao View Article