NUF2 Polyclonal antibody

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• KD/KO Validated

NUF2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 15731-1-AP Copied!

Publications (9)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

CDCA1, hNuf2, hNuf2R, hsNuf2, Kinetochore protein Nuf2, NUF2, NUF2R

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  WB analysis using 15731-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 15731-1-AP

  WB result of NUF2 antibody (15731-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUF2 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 15731-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 15731-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human testis using 15731-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 15731-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human skeletal muscle using 15731-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 15731-1-AP

  IP result of anti-NUF2 (IP:15731-1-AP, 3ug; Detection:15731-1-AP 1:500) with HEK-293 cells lysate 1760ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, human liver tissue, human testis tissue, PC-3 cells, human skeletal muscle tissue
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15731-1-AP targets NUF2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8479

  Product name: Recombinant human NUF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 105-464 aa of BC008489

  Sequence: ETADILCPKAKRTSRFLSGIINFIHFREACRETYMEFLWQYKSSADKMQQLNAAHQEALMKLERLDSVPVEEQEEFKQLSDGIQELQQSLNQDFHQKTIVLQEGNSQKKSNISEKTKRLNELKLSVVSLKEIQESLKTKIVDSPEKLKNYKEKMKDTVQKLKNARQEVVEKYEIYGDSVDCLPSCQLEVQLYQKKIQDLSDNREKLASILKESLNLEDQIESDESELKKLKTEENSFKRLMIVKKEKLATAQFKINKKHEDVKQYKRTVIEDCNKVQEKRGAVYERVTTINQEIQKIKLGIQQLKDAAEREKLKSQEIFLNLKTALEKYHDGIEKAAEDSYAKIDEKTAELKRKMFKMST

• Full Name: NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
• Calculated Molecular Weight: 464 aa, 54 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC008489
• Gene Symbol: NUF2
• Gene ID (NCBI): 83540
• RRID: AB_2154586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BZD4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. NUF2 was reported to be overexpressed in various kinds of carcinomas including lung cancer, cholangiocellular cancer, urinary bladder cancer, renal cell cancer, colorectal cancer, gastric cancer, ovarian cancer and serous adenocarcinoma. Elevated expression of NUF2 was associated with poorer prognosis of non-small cell lung cancer patients (PMID: 17079454). Knockdown of NUF2 significantly caused cell growth delay and increased apoptosis in cell lines of non-small cell lung cancer, colorectal cancer, gastric cancer and ovarian cancer.

Publications

What published studies show

The NUF2 Polyclonal antibody (Cat# 15731-1-AP) has 9 citations published in journals including Nature Communications, Advanced Science, Cell Death & Disease, International Journal of Biological Sciences, Biochemical Pharmacology, Scientific Reports, Carcinogenesis, Frontiers in Oncology, and Oncology Letters. Research fields span kinetochore-microtubule biology, mitotic checkpoint regulation, and multiple cancer types including renal cell carcinoma, prostate cancer, gallbladder cancer, ovarian cancer, and adenoid cystic carcinoma.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Nat Commun; LUBAC controls chromosome alignment by targeting CENP-E to attached kinetochores.; Authors - Min Wu
human	WB	Adv Sci (Weinh); CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC; Authors - Qi Li
human	WB	Cell Death Dis; LncRNA CDKN2B-AS1 stabilized by IGF2BP3 drives the malignancy of renal clear cell carcinoma through epigenetically activating NUF2 transcription.; Authors - Xina Xie
human	WB,IHC	Int J Biol Sci; NUF2 Drives Clear Cell Renal Cell Carcinoma by Activating HMGA2 Transcription through KDM2A-mediated H3K36me2 Demethylation; Authors - Jiatian Lin ; KD Validated
human	WB,IP,IHC	Biochem Pharmacol; NUF2 activated by YY1 promotes prostate cancer malignancy via p38/MAPK signaling axis and serves as a therapeutic target; Authors - Chengqi Jin
human	WB	Sci Rep; Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.; Authors - Hexiao Shen

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

One user (Katie W.) rated this product 4 out of 5 stars in May 2022. She used it for Western Blot at a 1:1000 dilution on T cells and reported that it "works well for western." Overall, the single review is positive, confirming reliable performance in WB applications.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Katie (Verified Customer) (05-23-2022)

Works well for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T cells