NUMB Monoclonal antibody

• Featured Product
• KD/KO Validated

NUMB Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

2G12B7

Cat no : 60137-1-Ig

Synonyms

h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171

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Validation Data Gallery

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  WB analysis of human brain using 60137-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60137-1-Ig (NUMB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 60137-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 60137-1-Ig (NUMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 60137-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60137-1-Ig (NUMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 60137-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60137-1-Ig (NUMB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of rat brain using 60137-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60137-1-Ig (NUMB Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 60137-1-Ig

  Immunofluorescent analysis of A431 cells using 60137-1-Ig (NUMB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Tested Applications

• Positive WB detected in: Human brain tissue,
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

60137-1-Ig targets NUMB in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: peptide
• Full Name: numb homolog (Drosophila)
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 66-72 kDa
• GenBank Accession Number: BC068476
• Gene Symbol: NUMB
• Gene ID (NCBI): 8650
• RRID: AB_10598775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can't recognize the NUMBL.

Publications

Species	Application	Title
rat	WB,IF	Sci Rep; Hepatic stem cell Numb gene is a potential target of Huang Qi Decoction against cholestatic liver fibrosis.; Authors - Wen Xu ; KD Validated
mouse	WB,IF,CoIP	Neurosci Lett; Numb deficiency causes impaired trafficking of mGlu5 in neurons and autistic-like behaviors.; Authors - Na Wang ; KO Validated
human	WB	Mol Biol Rep; Different roles of Numb-p72 and Numb-p65 on the trafficking of metabotropic glutamate receptor 5.; Authors - Na Wang
rat	WB	Stem Cell Res Ther; BM-MSCs overexpressing the Numb enhance the therapeutic effect on cholestatic liver fibrosis by inhibiting the ductular reaction; Authors - Yan-Nan Xu
human	IF	Cell Rep; Stabilizing vimentin phosphorylation inhibits stem-like cell properties and metastasis of hybrid epithelial/mesenchymal carcinomas; Authors - Nick A Kuburich