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  • KD/KO Validated

NUMBL Polyclonal antibody

NUMBL Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10111-1-AP

Synonyms

CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23



Tested Applications

Positive WB detected inmouse brain tissue, mouse spleen tissue, HeLa cells, mouse lung tissue, mouse liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10111-1-AP targets NUMBL in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUMBL fusion protein Ag0156
Full Name numb homolog (Drosophila)-like
Calculated molecular weight 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC001794
Gene symbol NUMBL
Gene ID (NCBI) 9253
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specifically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.

Protocols

Product Specific Protocols
WB protocol for NUMBL antibody 10111-1-APDownload protocol
IHC protocol for NUMBL antibody 10111-1-APDownload protocol
IF protocol for NUMBL antibody 10111-1-APDownload protocol
IP protocol for NUMBL antibody 10111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Mol Endocrinol

Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation.

Authors - Moran Tyler B TB
humanWB,IHC

J Clin Endocrinol Metab

PMID: 16705073

Authors - Sinha-Hikim Indrani I
humanWB,IHC

Cancer Res

Regulation of lung cancer metastasis by Klf4-Numb-like signaling.

Authors - Vaira Valentina V
  • KD Validated
mouseWB

Nat Neurosci

Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors.

Authors - Rasin Mladen-Roko MR