|Positive WB detected in||mouse brain tissue, mouse spleen tissue, HeLa cells, mouse lung tissue, mouse liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human brain tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-12 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10111-1-AP targets NUMBL in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NUMBL fusion protein Ag0156|
|Full Name||numb homolog (Drosophila)-like|
|Calculated molecular weight||65 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC001794|
|Gene ID (NCBI)||9253|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specifically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.
|Product Specific Protocols|
|WB protocol for NUMBL antibody 10111-1-AP||Download protocol|
|IHC protocol for NUMBL antibody 10111-1-AP||Download protocol|
|IF protocol for NUMBL antibody 10111-1-AP||Download protocol|
|IP protocol for NUMBL antibody 10111-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation.
J Clin Endocrinol Metab
Regulation of lung cancer metastasis by Klf4-Numb-like signaling.
Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors.