NUMBL Monoclonal antibody

• 

  WB analysis of HepG2 using 60009-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60009-1-Ig (NUMBL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

NUMBL Monoclonal Antibody for WB, ELISA

Cat No. 60009-1-Ig

Clone No.4C10C5

Publications(15)

Reviews (1)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IP: See 1 publications below

Product Information

60009-1-Ig targets NUMBL in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0156

  Product name: Recombinant human NUMBLIKE protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-280 aa of BC001794

  Sequence: MNKLRQSLRRRKPAYVPEASRPHQWQADEDAVRKGTCSFPVRYLGHVEVEESRGMHVCEDAVKKLKAMGRKSVKSVLWVSADGLRVVDDKTKDLLVDQTIEKVSFCAPDRNLDKAFSYICRDGTTRRWICHCFLALKDSGERLSHAVGCAFAACLERKQRREKECGVTAAFDASRTSFAREGSFRLSGGGRPAEREAPDKKKAEAAAAPTVAPGPAQPGHVSPTPATTSPGEKGEAGTPVAAGTTAAAIPRRHAPLEQLVRQGSFRGFPALSQKNSPFKR

• Full Name: numb homolog (Drosophila)-like
• Calculated Molecular Weight: 65 kDa
• GenBank Accession Number: BC001794
• Gene Symbol: NUMBL
• Gene ID (NCBI): 9253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic Acid-Ammonium Sulfate Precipitation
• UNIPROT ID: Q9Y6R0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Numb-like (Numbl or Nbl) binds Notch and is an intracellular modulator of Notch signaling pathway. Numbl contains a phosphotyrosine binding (PTB) domain that could bind the Y682 motif present in the cytoplasmic tail of APP. Deduced molecular weight of Numbl is 72 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Elife; Host-derived Lactobacillus plantarum alleviates hyperuricemia by improving gut microbial community and hydrolase-mediated degradation of purine nucleosides; Authors - Yang Fu
		Elife; Alpha protocadherins and Pyk2 kinase regulate cortical neuron migration and cytoskeletal dynamics via Rac1 GTPase and WAVE complex in mice.; Authors - Li Fan
Mouse	WB	Commun Biol; STAT1 mediates the pro-inflammatory role of GBP5 in colitis; Authors - Yichen Li
	WB	Cells; PBRM1 Cooperates with YTHDF2 to Control HIF-1α Protein Translation.; Authors - Alena Shmakova
	WB	Oncol Lett; DNMT3B regulates proliferation of A549 cells through the microRNA-152-3p/NCAM1 pathway.; Authors - Bo Yang
human	WB	J Oncol; Plumbagin Enhances the Radiosensitivity of Tongue Squamous Cell Carcinoma Cells via Downregulating ATM.; Authors - Shu-Ting Pan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (01-13-2023)

Second band was found in some samples.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Drosophila ovaries

NUMBL Antibody Western Blot validation (1:5000 dilution) in Drosophila ovaries (Cat no:60009-1-Ig)