NUMBL Monoclonal antibody
NUMBL Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
5F6E1
Cat no : 66155-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells |
Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-12 cells, L02 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66155-1-Ig targets NUMBL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | NUMBL fusion protein Ag16771 |
Full Name | numb homolog (Drosophila)-like |
Calculated Molecular Weight | 65 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | BC001794 |
Gene Symbol | NUMBL |
Gene ID (NCBI) | 9253 |
RRID | AB_2881551 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specfically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.
Protocols
Product Specific Protocols | |
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WB protocol for NUMBL antibody 66155-1-Ig | Download protocol |
IHC protocol for NUMBL antibody 66155-1-Ig | Download protocol |
IF protocol for NUMBL antibody 66155-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |