NUMBL Monoclonal antibody

NUMBL Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5F6E1

Cat no : 66155-1-Ig

Synonyms

CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23

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Validation Data Gallery

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  WB analysis of L02 using 66155-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66155-1-Ig (NUMBL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66155-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66155-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66155-1-Ig (NUMBL antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 66155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66155-1-Ig (NUMBL antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-12 using 66155-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66155-1-Ig (NUMBL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of L02 using 66155-1-Ig

  Immunofluorescent analysis of L02 cells using 66155-1-Ig (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Tested Applications

• Positive WB detected in: L02 cells
• Positive IHC detected in: mouse brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-12 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66155-1-Ig targets NUMBL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NUMBL fusion protein Ag16771
• Full Name: numb homolog (Drosophila)-like
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC001794
• Gene Symbol: NUMBL
• Gene ID (NCBI): 9253
• RRID: AB_2881551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specfically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.