NUMBL Monoclonal antibody

NUMBL Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

5F6E1

Cat no : 66155-1-Ig

Synonyms

CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23



Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66155-1-Ig targets NUMBL in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NUMBL fusion protein Ag16771
Full Name numb homolog (Drosophila)-like
Calculated molecular weight 65 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC001794
Gene symbol NUMBL
Gene ID (NCBI) 9253
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specfically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.

Protocols

Product Specific Protocols
WB protocol for NUMBL antibody 66155-1-IgDownload protocol
IHC protocol for NUMBL antibody 66155-1-IgDownload protocol
IF protocol for NUMBL antibody 66155-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols