NUP107 Polyclonal antibody

• 

  WB analysis using 19217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19217-1-AP (NUP107 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19217-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19217-1-AP (NUP107 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 19217-1-AP

  IP result of anti-NUP107 (IP:19217-1-AP, 4ug; Detection:19217-1-AP 1:2000) with HeLa cells lysate 1320 ug.

NUP107 Polyclonal Antibody for WB, IP, ELISA

Cat No. 19217-1-AP

Publications(12)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

107 kDa nucleoporin, nucleoporin 107kDa, Nucleoporin Nup107, NUP107, NUP84

---

Tested Applications

• Positive WB detected in: COLO 320 cells, mouse testis tissue, HeLa cells, rat testis tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

19217-1-AP targets NUP107 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5250

  Product name: Recombinant human NUP107 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-338 aa of BC017167

  Sequence: MSMFSDFLQSFLKHSSSTVFDLVEEYENICGSQVNILSKIVSRATPGLQKFSKTASMLWLLQQEMVTWRLLASLYRDRIQSALEEESVFAVTAVNASEKTVVEALFQRDSLVRQSQLVVDWLESIAKDEIGEFSDNIEFYAKSVYWENTLHTLKQRQLTSYVGSVRPLVTELDPDAPIRQKMPLDDLDREDEVRLLKYLFTLIRAGMTEEAQRLCKRCGQAWRAATLEGWKLYHDPNVNGGTELEPVEGNPYRRIWKISCWRMAEDELFNRYERAIYAALSGNLKQLLPVCDTWEDTVWAYFRVMVDSLVEQEIQTSVATLDETEELPREYLGANWTL

• Full Name: nucleoporin 107kDa
• Calculated Molecular Weight: 106 kDa
• Observed Molecular Weight: 107 kDa
• GenBank Accession Number: BC017167
• Gene Symbol: NUP107
• Gene ID (NCBI): 57122
• RRID: AB_10597702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P57740
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Commun; The master energy homeostasis regulator PGC-1α exhibits an mRNA nuclear export function; Authors - Simeon R Mihaylov
rat	WB	Neuron; Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.; Authors - Zhixiong Liu
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
		Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
mouse	WB	Elife; Suppression of C9orf72 RNA repeat-induced neurotoxicity by the ALS-associated RNA-binding protein Zfp106.; Authors - Barbara Celona

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Barbara (Verified Customer) (03-28-2022)

Reliable antibody for western blot detection of NUP-107

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neuro-2A lysates