NUP133 Polyclonal antibody

NUP133 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 12405-1-AP

Synonyms

133 kDa nucleoporin, hNUP133, nucleoporin 133kDa, Nucleoporin Nup133, NUP133



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12405-1-AP targets NUP133 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUP133 fusion protein Ag3100
Full Name nucleoporin 133kDa
Calculated Molecular Weight 1156 aa, 129 kDa
Observed Molecular Weight 129 kDa
GenBank Accession NumberBC020107
Gene Symbol NUP133
Gene ID (NCBI) 55746
RRIDAB_2154450
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear pore complex protein Nup133 is a protein that in humans is encoded by the NUP133 gene. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.

Protocols

Product Specific Protocols
WB protocol for NUP133 antibody 12405-1-APDownload protocol
IHC protocol for NUP133 antibody 12405-1-APDownload protocol
IP protocol for NUP133 antibody 12405-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Int J Mol Sci

Fetal Zone Steroids and Estrogen Show Sex Specific Effects on Oligodendrocyte Precursor Cells in Response to Oxidative Damage.

Authors - Donna Elizabeth Sunny
humanChIP

Mol Cell Pediatr

Nup133 and ERα mediate the differential effects of hyperoxia-induced damage in male and female OPCs.

Authors - Donna Elizabeth Sunny